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| 庆大霉素高产菌株的抗性方法选育 | |
| 引用本文： | 吴健,张少平,戴桂馥,李宗伟,袁天阳,段广才.庆大霉素高产菌株的抗性方法选育[J].信阳师范学院学报(自然科学版),2007,20(2):191-194. |
| 作者姓名： | 吴健 张少平 戴桂馥 李宗伟 袁天阳 段广才 |
| 作者单位： | 1. 郑州大学,生物工程系,河南,郑州,450052;郑州大学,公共卫生学院,河南,郑州,450052 2. 郑州大学,生物工程系,河南,郑州,450052 3. 郑州大学,物理工程学院,河南,郑州,450052 4. 开封制药厂,河南,开封475003 5. 郑州大学,公共卫生学院,河南,郑州,450052 |
| 摘 要： | 以生产菌株绛红小单孢菌为出发株,应用N 离子注入及紫外线诱变技术,利用高浓度的庆大霉素梯度平板筛选,或配合含有高浓度庆大霉素的液体培养基的生态驯化,筛选到摇瓶效价分别比出发株提高75.2%和70.1%的N08和Wt63两个高产菌株,10 L发酵罐发酵产量分别达2 118 u/mL和1 843 u/mL,C组分含量符合中国药典2000版的规定. |
| 关 键 词： | 绛红小单孢菌 庆大霉素 育种 诱变 |
| 文章编号： | 1003-0972（2007）02-0191-04 |
| 收稿时间： | 2006-09-29 |
| 修稿时间： | 2007-01-10 |
Breeding of Gentamicin High Yield Strains from Micromonospora Purpurea through Gentamicin Resistance Selection |
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| WU Jian,ZHANG Shao-ping,DAI Gui-fu,LI Zong-wei,YUAN Tian-yang,DUAN Guang-cai.Breeding of Gentamicin High Yield Strains from Micromonospora Purpurea through Gentamicin Resistance Selection[J].Journal of Xinyang Teachers College(Natural Science Edition),2007,20(2):191-194. | |
| Authors: | WU Jian ZHANG Shao-ping DAI Gui-fu LI Zong-wei YUAN Tian-yang DUAN Guang-cai |
| Abstract: | Two high yield gentamicin strains of Micromonospora purpurea were screened by agar plate or/and liquid medium composed of high concentration of gentamicin after implanted by suitable doses of N at 30 KeV and exposed by UV.The yields of these two strains were 75.1% and 70.2% higher than that of the original strain,respectively.And the gentamicin C component is qualified to the Medicine Code of China 2000. |
| Keywords: | Micromonospora purpurea gentamicin breeding mutation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |