家蚕非编码RNA Bm-152功能初探

Bm-152是前期研究中发现的一个在家蚕丝腺中高表达的非编码RNA.采用实时荧光定量PCR技术对Bm-152在家蚕5龄到预蛹期不同发育天数丝腺中的表达谱进行检测.同时,在个体中过表达Bm-152,检测Bm-152可能参与的信号通路.结果显示,Bm-152在家蚕5龄幼虫丝腺发育过程中无明显的变化规律,但在吐丝第1天表达量较高,随后表达量又下降.通过在血淋巴中注射2μg piggyBacA3-EGFP-A3-Bm-152]过表达载体,48h后发现Bm-152在丝腺组织中无明显过表达,但在非丝腺组织中可实现明显过表达,且表达量上调1.66倍;蜕皮激素信号通路基因Usp在非丝腺组织中上调1.95倍,E75A在非丝腺组织中上调2.26倍,保幼激素信号通路基因Met在非丝腺组织中上调1.79倍.表明非编码RNA Bm-152可能通过蜕皮激素和保幼激素信号通路参与家蚕发育,该结果为进一步探索Bm-152的功能提供了方向.

Preliminary Functional Analysis of Noncoding RNA Bm-152 of Bombyx Mori

Non-coding RNA Bm-152 accumulated more in silk gland of silkworm Bombyx mori.Real-time PCR was used to study the expression levels of Bm-152 in the silk glands of silkworm from the fifth instar larvae to prepupa stage.Furthermore,the signaling pathway in which Bm-152 may participate were studied as Bm-152 was overexpressed in the 3 r d day of the fifth larva of silkworm.Results showed that Bm-152 had no obvious change during the development of silk glands of the fifth instar larvae of silkworm,Bm-152 had more expression at the first spinning day in the silk gland,then decreased in the pre-pupa stage.After 48 hinjection of 2μg dose of piggyBacA3-EGFP-A3-Bm-152]overexpression vector,it was found that Bm-152 was not overexpressed in the silk gland,but was significantly overexpressed in the non-silk gland,and the expression of Bm-152 increased1.66 times.Moreover,the expression of Usp(ecdysone signaling pathway)increased by 1.95 times in the nonsilkgland tissues,E75 A(ecdysone signaling pathway)increased by 2.26 times,and the expression of Met(juvenile hormone signaling pathway)increased by 1.79 times in the non-silkgland tissues.Bm-152 might participate in the development of silkworm through the ecdysone and juvenile hormone signaling pathway.These results laid foundations for further study of functions of Bm-152 in silkworm.