| 高效液相色谱法测定蓝耳草中β-蜕皮激素含量 |
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| 引用本文： | 徐慧.高效液相色谱法测定蓝耳草中β-蜕皮激素含量[J].广西科学院学报,2010,26(3):239-241. |
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| 作者姓名： | 徐慧 |
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| 作者单位： | 广西分析测试研究中心,广西南宁,530022 |
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| 摘 要： | 以甲醇为溶剂,用索式提取法提取蓝耳草Cyanotis vaga(Lour.)Roem.et Schult]中β-蜕皮激素,用高效液相色谱法(HPLC)测定其含量。结果,β-蜕皮激素在0.4～8.0μg范围内线性关系良好,r2=0.9992,平均回收率为99.31%,RSD值均小于3%。
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| 关 键 词： | 高效液相色谱法 β-蜕皮激素 蓝耳草 提取工艺 |
| 收稿时间： | 2010/5/11 0:00:00 |
Determination of β-Ecdysone in Cyanotis vaga(Lour.)Roem.et Schult by HPLC |
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| XU Hui.Determination of β-Ecdysone in Cyanotis vaga(Lour.)Roem.et Schult by HPLC[J].Journal of Guangxi Academy of Sciences,2010,26(3):239-241. |
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| Authors: | XU Hui |
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| Institution: | XU Hui(Guangxi Research Center of Analysis and Testing,Nanning,Guangxi,530022,China) |
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| Abstract: | Using methanol as the solvent extraction rate,the β-Ecdysone in Cyanotis vaga（Lour.） Roemet Schult is extracted by the soxhlet extraction method.The content of β-Ecdysone is determined by HPLC.A linear curve of β-Ecdysone was obtained in the range of 0.4～0.8μg with r2 equalling to 0.9992.The average recovery was 99.31% with RSD being less than 3%. |
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| Keywords: | HPLC β-Ecdysone Cyanotis vaga（Lour ） Roem et Schult extraction process |
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