超高效液相色谱法测定露酒中的枸橼酸西地那非

为了建立露酒中非法添加枸橼酸西地那非的一种快速、准确、灵敏高的专属性检测方法,采用反相超高效液相色谱法,以C18柱(2.1mm×100mm,1.7μm),柱温30℃,检测波长293.6nm,流动相为乙腈∶水∶冰醋酸∶三乙胺(35∶65∶0.8∶0.4;V/V),流速0.15ml/min的条件进行实验。结果枸橼酸西地那非在0.05～50μg/ml范围内线性关系良好,相关系数r=0.9998,平均回收率为98.93%,RSD=1.86%。建立的方法准确、可靠,可以用于露酒中枸橼酸西地那非的含量检测。

Detection of Sildenafil Citrate in the Liqueur by Ultra Performance Liquid Chromatography

In order to estabish a quick,accurate,sensitive method for analysis of sildenafil citrate,which illegally added into the liqueur,reversed phase ultra performance liquid chromatography was used.The column was C18 2.1 ×100mm.Column temperature was 30℃.The mobile phase was acetonitrile∶water∶acetic acid∶triethylamine（35∶65∶0.8∶0.4;V/V） with flow rate 0.15 ml/min and the detection wavelength was 293.6 nm.The linear range of the calibration curve of sildenafil citrate were 0.05～50μg/ml（r=0.9998）.The mean rates of recovery was 98.93% and the relative standard deviations（n=6） was 1.86%.The method is accurate,realiable that can be used to determine sildenfil citrate in the liqueur.