大枣多糖的提取与抗活性氧研究

为了分析大枣多糖对各类活性氧的清除能力,建立了热水提取-乙醇沉淀-氯仿、正丁醇去蛋白的工艺以提取大枣多糖(JPS),并作了糖的含量测定.用化学发光分析法分别测定提取的JPS对全血化学发光法中的全血白细胞呼吸爆发中产生的活性氧(H2O2、O2(-·)、·OH)、连苯三酚自氧化法产生的O2(-·)(超氧阴离子自由基)、抗坏血酸-Cu2+-H2O2体系产生的·OH(羟氧自由基)以及H2O2-鲁米诺发光体系中的H2O2的清除作用.结果:大枣多糖的得率为0.848%,总糖含量为37.7% .测定JPS清除·OH、O2(-·)、H2O2、白细胞呼吸爆发中产生的氧自由基活性的AOV值分别为109.48、9057.9 、312.01、5.204.结论:JPS具有清除氧自由基的作用,其活性大小与多糖的用量呈正相关.在全血生理环境下,对全血化学发光中活性氧的清除能力最强.

Extraction of Jujube Ploysaccharide and Its Effect of Cleaning Oxygen Radical

To isolate and extract jujube polysaccharide from jujube and analyse its effect on cleaning all kinds of oxygen radical,a technique with hot water extract, ethanol sedimentation, chloroform and butanol deproteinization was preformed. Sugar content was also determined. With the help of chemiluminescent analysis,we separately determined JPS cleaning activities to oxygen free radicals of H₂O₂,O₂^·-, ·OH in whole blood from Chemiluminescence, O₂^·- from Pyrogallol autooxidation method, ·OH from ascorbic acid Cu²⁺ -H₂O₂ system, H₂O₂ from Hydrogen peroxide -luminous system. The extraction ratio was 0.848%.Total sugar content was 37.7%. AOV was 109.48 for ·OH, 9057.9 for O₂^·-, 312.01, for H₂O₂, and 5.204 for oxygen free radicals from phagocytic of leucocyte in whole blood chemiluminescence. The test showed that JPS could weed out oxygen radical, and its effect increaseed with amount of suger increasing.JPS has strongest ability in clearing up oxygen free radical from chemiluminescence in whole blood under the circumstance of whole blood physiology.