论鸡传染性法氏囊病病毒细胞苗的制造和应用

目前使用的多种疫苗仍未能有效控制鸡传染性法氏囊病（ＩＢＤ）的发生，原因是疫苗质量难以保证和免疫程序或方法不发。解决的办法：病毒培养宜选用无特定病原（ＳＰＦ）鸡胚或非免疫健康鸡群的鸡胚，消化时间控制在１５ｍｉｎ左右，细胞数在１００－１５０万／ｍＬ，接毒量为０．１％－０．２％，小牛血清使用浓度为３－５％；疫苗冻干过程应在不断搅拌病毒培养液的同时控制加入保护剂的速度。免疫程序：无母源抗体或母源抗体不清雏

On the Production and Application of the Vaccine of the Infectious Bursal Disease Virus

At present, Infectious Bursal Disease (IBD) is widespread epidemic and results in serious damage to the poultry of China, although several kinds of vaccines are applied. It''s the main reason that the quality of the vaccine and the order or method of the immunity are inappropriate. The existing problems and the effective resolving measures are put forward in the manufacturing operation and application of vaccine:Chick embryo without maternal antibody (MA) must be chosen, Time of digestion is about 15 minutes, Cell number is 1~1.5 million/mL, Dose of inoculated virus''s seed is 0.1%-0.2% culture solution included 3%-5% serum of newborn calf, and the speed is dominated when the culture solution of virus and protective agent are mixed before freeze-dried. For young chickens without MA or little being known about MA, the immunity are executed respectively in 7, 14 and 25-old-day chickens, but for young chickens having MA in 10-14, 21 and 40-old-day respectively with nasal drops or eye drops. The dose of common practice of vaccine is one million PFu per chicken, the minimum is a hundred thousand PFu.