| 丙酮丁醇梭菌发酵糖蜜生产丁醇的发酵条件优化 |
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| 引用本文： | 米慧芝,杨登峰,关妮,苏海锋,黄日波.丙酮丁醇梭菌发酵糖蜜生产丁醇的发酵条件优化[J].广西科学,2011,18(3):278-282,288. |
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| 作者姓名： | 米慧芝 杨登峰 关妮 苏海锋 黄日波 |
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| 作者单位： | 广西科学院非粮生物质酶解国家重点实验室,国家非粮生物质能源工程技术研究中心,广西生物炼制重点实验室,广西南宁 530007 |
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| 摘 要： | 运用单因素试验结合正交设计法优化丙酮丁醇梭菌发酵甘蔗糖蜜生产丁醇的发酵条件,并进行验证试验.得到发酵条件的最优组合为(NH4)2HPO4的添加量为0.3％,H2NCONH2的添加量为0.1％、Na2 HPO4的添加量为0.5％,KH2 PO4的添加量为0.5％,L-半胱氨酸盐酸盐-水物的添加量为0.025％,蛋白胨的添...
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| 关 键 词： | 丙酮丁醇梭菌 甘蔗糖蜜 丁醇 正交试验 |
| 收稿时间： | 2011/1/7 0:00:00 |
Butanol Yield from Sugarcane Molasses by Clostridium acetobutylicum and Optimization of Its Fermentation Conditions |
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| MI Hui-zhi,YANG Deng-feng,GUAN Ni,SU Hai-feng and HUANG Ri-bo.Butanol Yield from Sugarcane Molasses by Clostridium acetobutylicum and Optimization of Its Fermentation Conditions[J].Guangxi Sciences,2011,18(3):278-282,288. |
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| Authors: | MI Hui-zhi YANG Deng-feng GUAN Ni SU Hai-feng and HUANG Ri-bo |
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| Institution: | MI Hui-zhi,YANG Deng-feng,GUAN Ni,SU Hai-feng,HUANG Ri-bo(State Key Laboratory of Non-food Biomass and Enzyme Technology,National Engineering Research Center for Non-food Biorefinery,Guangxi Academy of Sciences,Guangxi Key Laboratory of Biorefinery,Nanning,Guangxi,530007,China) |
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| Abstract: | Using single-fador experiments combined with orthogonal design qitimization the fermentation conditions,which the clostridium aletobutylicum ferment sugercace molasses to producte batand and verification test.The obtained optimal combination of nutrients is 0.3%(NH4) 2HPO4,0.1%H2NCONH2,0.5% Na2HPO4,0.5% KH2PO4,0.025% L-cysteine hydrochloride monohydrate,0.9% tryptone,and 0.1% NaH2PO4.The results show that the butanol yield in this optimized fermentation reaches 11.0536g/L,which increased about 23.58% compar... |
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| Keywords: | Clostridium acetobutylicum sugarcane molasses butanol orthogonal test |
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