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| 高压脉冲电场提取猪皮胶原蛋白 | |
| 引用本文： | 韦汉昌,韦群兰,韦善清.高压脉冲电场提取猪皮胶原蛋白[J].广西科学,2011,18(3):235-237. |
| 作者姓名： | 韦汉昌 韦群兰 韦善清 |
| 作者单位： | 1. 广西师范大学,广西桂林541004;南宁地区教育学院,广西南宁530001 2. 南宁地区教育学院,广西南宁,530001 3. 广西大学,广西南宁,530004 |
| 基金项目： | 广西自然科学基金项目(桂科基0575055)资助 |
| 摘 要： | 为了研究高压脉冲电场(PEF)对提取猪皮胶原蛋白的影响,在料液比1:10、枯草杆菌中性蛋白酶用量400U/g、酶解温度40℃、pH值6.5的条件下,分别改变电场强度、脉冲频率和酶解时间,用考马斯亮兰法测定胶原蛋白的浓度并计算其溶出率.结果表明,最适电场强度为25kV· cm-1、脉冲频率为40Hz、脉冲时间1s、酶解时... |
| 关 键 词： | 胶原蛋白 猪皮 提取 高压脉冲电场 |
| 收稿时间： | 2011/2/21 0:00:00 |
Extracting Collagen from Pigskin by PEF |
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| WEI Han-chang,WEI Qun-lan and WEI Shan-qing.Extracting Collagen from Pigskin by PEF[J].Guangxi Sciences,2011,18(3):235-237. | |
| Authors: | WEI Han-chang WEI Qun-lan and WEI Shan-qing |
| Institution: | WEI Han-chang1,2,WEI Qun-lan2,WEI Shan-qing3(1.Guangxi Normal University,Guilin,Guangxi,541004,China,2.Nanning Prefecture Education College,Nanning,530001,3.Guangxi University,530004,China) |
| Abstract: | The effect of PEF on the yield of extracting collagen from pigskin was studied.The experimental processes were that preprocessed pigskin was treated by PEF at first,hydrolyzed by bacillus subtilis neutral proteinase followed,and the dissolution ratio of collagen was tested by Bradford Method at last.The results showed that the dissolution ratio of the extracting method preprocessed by PEF could be more higher than that of un-preprocessed one,and the preprocessed method could save more enzymolysis time.The d... |
| Keywords: | collagen pigskin extraction PEF |
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