光声光谱技术在光合作用研究中的应用

当光照射密闭容器里的样品时,容器内能产生声波,这一现象称为光声效应,光声光谱是基于光声效应的一种光谱技术。由于光声光谱技术本身的特点,光合作用研究领域越来越多地应用该技术研究光合作用的机理,比如：光谱扫描、光声能量储存的性质、环式电子传递、状态转变与爱默生效应、光反应中心的原初光化学过程、光声瞬态研究、环境胁迫等。光声光谱技术在光合作用研究中的应用,开拓了视野,丰富了对光合作用机理的认识。

The Application of Photoacoustic Spectroscopy in the Research of Photosynthesis

Photoacoustic spectroscopy technique has been introduced to photosynthetic research since the 1970's.Compared with oxygen electrode,PA technique could probe the kinetics of oxygen evolution more sensitively and accurately.PA technique is now a convenient and frequently used method in study of the state transitions and cyclic electron transport activity.The energy transfer efficiency of PSI could be measured by PA technique.Photoacoustic signals detected by hydrophone in water could reveal the micro-volume change occurred during the primary photochemistry reaction.The"Depth Analysis"of photoacoustic spectrometer could be used to scan the light absorption spectra of tissue layer under different depth of sample's surface.This paper summarize the application of Photoacoustic spectroscopy technique in the research of photosynthesis.