CD40在支气管哮喘患者外周血嗜酸性粒细胞的表达

探讨过敏哮喘患者和正常人外周血嗜酸性粒细胞（EOS）表达CD40的差异，采集10例过敏性哮喘患者和8例正常对照者静脉血用于纯化EOS，以流细胞仪检测CD40在EOS表达的表达，EOS胞浆中的CD40蛋白则以铭疫细胞化学方法显示，结果表明，哮喘患者EOS表面存在CD40表达，但CD40并不表达于正常人EOS的表面，无论是哮喘患者还是正常人，其EOS的胞浆均可检测到CD40蛋白，人EOS均能合成和储存CD40，CD40分子可以移行到哮喘患者EOS的表达而不能移行到正常人EOS的表面，揭示CD40在哮喘的发病机理中具有某种作用。

CD40 Expression by Peripheral Blood Eosinophils from Asthmatics *

To identify the differences in CD40 expression on the eosinophils from patients with allergic asthma and healthy people,the purified eosinophils were isolated from 10 allergic asthmatics and 8 non allergic normal subjects. Flow cytometry was used to detect the surface expression of CD40 on eosinophils and immunocytochemical techniques were used to determine the intracellular CD40 expression in eosinophils. Intracellular CD40 expression was observed in the eosinophils from both allergic asthmatics and normal donors. The eosinophils from normal donors lacked CD40 surface expression;in contrast,the eosinophils from allergic asthmatics were all positive for CD40 surface expression. It could be concluded that human eosinophils appear uniformly to contain CD40, but vary in the levels of CD40 expressed on the outer plasma membrane with expression enhanced on the eosinophils from allergic asthmatics.