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方格星虫多糖碱法提取工艺研究
引用本文:许明珠,张琴,童潼,于海瑞,董兰芳.方格星虫多糖碱法提取工艺研究[J].广西科学,2014,21(2):164-168.
作者姓名:许明珠  张琴  童潼  于海瑞  董兰芳
作者单位:[1]广西壮族自治区海洋研究所广西海洋生物技术重点实验室,广西北海536000 [2]潍坊学院生物与农业工程学院、山东省高校生物化学与分子生物学重点实验室潍坊学院潍坊高新区天润生物工程研发中心,山东潍坊261061
基金项目:广西科学研究与技术开发计划(桂科攻11107011-6);广西科学院基本科研业务费项目(12YJ25HYS20)资助.
摘    要:【目的】研究碱法提取方格星虫体壁中水溶性方格星虫多糖的条件及优化。【方法】根据料液比、浸提时间、浸提温度、碱液浓度对多糖得率的影响,并通过正交试验得到方格星虫水溶性多糖浸提工艺优选因素组合。【结果】影响方格星虫多糖得率的因素主次顺序为浸提温度料液比浸提时间碱液浓度;最佳浸提条件为温度50℃,料液比1∶6g/mL,时间4h,碱液(NaOH)的质量分数为8%。【结论】最佳浸提工艺条件下多糖浸提提取率最大为1.81%。

关 键 词:方格星虫  多糖  碱提  优化
收稿时间:2013/9/4 0:00:00
修稿时间:2013/10/11 0:00:00

Optimization of Alkaline Extraction for Polysaccharide from Sipunculus nudus Linnaeus
XU Ming-zhu,ZHANG Qin,TONG Tong,YU Hai-rui and DONG Lan-fang.Optimization of Alkaline Extraction for Polysaccharide from Sipunculus nudus Linnaeus[J].Guangxi Sciences,2014,21(2):164-168.
Authors:XU Ming-zhu  ZHANG Qin  TONG Tong  YU Hai-rui and DONG Lan-fang
Institution:1. Guangxi Institute of Oceanology, Key Laboratory of Marine Biotechnology of Guangxi, Beihai,Guangxi, 536000,China 2. Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong (Weifang University), College of Biological and Agricultural Engi- neering,Weifang University,Tianrun Bioengineering R& D Center of Weifang New and High- tech Zone, Weifang, Shandong, 261061, China)
Abstract:Objective]To choose the optimum alkaline extraction process of water-soluble poly-saccharide from Sipunculus nudus Linnaeus.Methods]The orthogonal test design with four factors was used to study the effect of the ratio of material to fluid,extraction time,extraction temperature and alkali concentration on the extracting rate of water-soluble polysaccharides from S.nudus .Results]The order of the factors that affect the yield ofS.nudus polysaccharide is extraction temperature〉ratio of material to fluid〉 extraction time〉 alkali concentration;the optimum extraction conditions were temperature 50℃,ratio of material to fluid 1 ∶ 6 g /mL,time 4h and alkali (NaOH)content of 8%.Conclusion]Under the above conditions,the optimum extraction rate is 1.81%.
Keywords:Sipunculus nudus  polysaccharide  alkaline extraction  optimizing
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