| 产丁醇大肠杆菌工程菌的构建 |
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| 引用本文： | 林丽华,郭媛,裴建新,庞浩,黄日波.产丁醇大肠杆菌工程菌的构建[J].广西科学,2014,21(1):42-46. |
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| 作者姓名： | 林丽华 郭媛 裴建新 庞浩 黄日波 |
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| 作者单位： | 广西科学院;非粮生物质酶解国家重点实验室;国家非粮生物质能源工程技术研究中心;广西生物质产业化工程院;广西生物炼制重点实验室;广西大学生命科学与技术学院; |
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| 基金项目： | 广西科技合作与交流计划项目(桂科合1347004-1);广西科技攻关项目(桂科攻10123007-3);广西科学院基本科研业务费项目(12YJ25SW05、13YJ22SW02);国家自然科学基金(21366007)资助 |
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| 摘 要： | 【目的】为了在大肠杆菌(Escherichia coli)中导入改良的丁醇合成途径,使非生产菌株大肠杆菌具备产丁醇的能力。【方法】克隆大肠杆菌乙酰转移酶基因atoB和丙酮丁醇梭菌(Clostridium acetobutylicum)丁醇合成途径关键酶基因(crt、hbd、adhE),构建多顺反子表达质粒pSE380-atoB-adhE-crt-hbd;克隆齿垢密螺旋体(Treponema denticola)反式烯酰辅酶A还原酶基因ter,构建表达质粒pSTV29-ter,并将双质粒导入到大肠杆菌。【结果】构建的工程菌能半厌氧发酵产微量丁醇,产量为0.08g/L。【结论】大肠杆菌中的丁醇合成途径导入成功,构建了产丁醇的大肠杆菌工程菌。
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| 关 键 词： | 正丁醇 大肠杆菌 丙酮丁醇梭菌 齿垢密螺旋体 半厌氧发酵 |
| 收稿时间： | 2013/8/5 0:00:00 |
| 修稿时间： | 2013/8/26 0:00:00 |
The Construction of Recombinant Escherichia coli Producing Butanol |
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| LIN Li-hu,GUO Yuan,PEI Jian-xin,PANG Hao and HUANG Ri-bo.The Construction of Recombinant Escherichia coli Producing Butanol[J].Guangxi Sciences,2014,21(1):42-46. |
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| Authors: | LIN Li-hu GUO Yuan PEI Jian-xin PANG Hao and HUANG Ri-bo |
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| Institution: | Guangxi Academy of Sciences, State Key Laboratory of Non-Food Biomass and Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Biomass Industrialization Engineering Institute, Guangxi Key Laboratory of Biorefinery, Nanning, Guangxi, 530007, China;College of Life Science & Technology of Guangxi University,Guangxi Academy of Sciences, State Key Laboratory of Non-Food Biomass and Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Biomass Industrialization Engineering Institute, Guangxi Key Laboratory of Biorefinery, Nanning, Guangxi, 530007, China,Guangxi Academy of Sciences, State Key Laboratory of Non-Food Biomass and Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Biomass Industrialization Engineering Institute, Guangxi Key Laboratory of Biorefinery, Nanning, Guangxi, 530007, China,Guangxi Academy of Sciences, State Key Laboratory of Non-Food Biomass and Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Biomass Industrialization Engineering Institute, Guangxi Key Laboratory of Biorefinery, Nanning, Guangxi, 530007, China;College of Life Science & Technology of Guangxi University and Guangxi Academy of Sciences, State Key Laboratory of Non-Food Biomass and Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Biomass Industrialization Engineering Institute, Guangxi Key Laboratory of Biorefinery, Nanning, Guangxi, 530007, China;College of Life Science & Technology of Guangxi University |
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| Abstract: | Objective] A modified 1-butanol pathway was constructed in non-native producer Escherichia coli to produce butanol.Method] By cloning the acetyltransferase gene from E.coli, and the key butanol synthetic pathway genes crt, hbd and adhE from Clostridium acetobutylicum, the polycistron expression plasmid pSE380-atoB-adhE-crt-hbd was constructed;by cloning the trans-2-enoyl-CoA reductase gene ter from Treponema denticola, the expression plasmid pSTV29-ter was constructed.Result] The recombinant E.coli containing the double plasmids was fermented in micro-aerobic and the maximal concentration of butanol was 0.08g/L at 24h.Conclusion] The butanol synthetic pathway was expressed in E.coli and the recombinant strain of producing butanol was constructed successfully. |
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| Keywords: | 1-butanol Escherichia coli Clostridium acetobutylicum Treponema denticola micro-aerobic fermentation |
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