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柳珊瑚共生菌Bucillus methylotrophicus发酵液化学成分研究
引用本文:龙彬,高程海,胡丽琴,潘丽霞,林琳,余炼,文良娟.柳珊瑚共生菌Bucillus methylotrophicus发酵液化学成分研究[J].广西科学,2014,21(1):89-92.
作者姓名:龙彬  高程海  胡丽琴  潘丽霞  林琳  余炼  文良娟
作者单位:广西科学院广西近海海洋环境科学重点实验室;广西大学轻工与食品工程学院;
基金项目:国家自然科学基金项目(81260480);广西自然科学基金项目(2011GXNSFB018035,2011GXNSFE018002,2012GXNSFAA053160,2012GXNSFEA053001)资助
摘    要:【目的】研究柳珊瑚Anthogorgia caerulea共生细菌Bacillus methylotrophicus发酵液中的化学成分。【方法】采用多种柱色谱和高效液相色谱分离技术,对柳珊瑚A.caerulea共生菌B.methylotrophicus发酵液中化学成分进行研究,运用理化和波谱分析方法鉴定其化学结构。【结果】从共生细菌B.Methylotrophicus发酵液中分离了6个化合物,分别鉴定为环(脯-甘)二肽(1)、环(S-脯-R-亮)二肽(2)、胸苷(3)、尿苷(4)、原儿茶酸(5)、3-Furancarboxylic acid-5-(hydroxmenthy)-Furan(6)。【结论】化合物1~6均是首次从该种细菌中分离得到,丰富了柳珊瑚共生菌B.methylotrophicus的化学多样性。

关 键 词:柳珊瑚  海洋细菌  化合物  结构鉴定
收稿时间:2013/11/1 0:00:00
修稿时间:2013/12/9 0:00:00

Study on Chemical Constituents from Marine Gorgonian-associated Bacterium Bucillus methylotrophicus
LONG Bin,GAO Cheng-hai,HU Li-qin,PAN Li-xi,LIN Lin,YU Lian and WEN Liang-juan.Study on Chemical Constituents from Marine Gorgonian-associated Bacterium Bucillus methylotrophicus[J].Guangxi Sciences,2014,21(1):89-92.
Authors:LONG Bin  GAO Cheng-hai  HU Li-qin  PAN Li-xi  LIN Lin  YU Lian and WEN Liang-juan
Institution:Guangxi Key Laboratory of Maine Environmental Science, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;College of Light Industry and Food Engineering, Guangxi University, Nanning, Guangxi, 530004, China,Guangxi Key Laboratory of Maine Environmental Science, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China,Guangxi Key Laboratory of Maine Environmental Science, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;College of Light Industry and Food Engineering, Guangxi University, Nanning, Guangxi, 530004, China,Guangxi Key Laboratory of Maine Environmental Science, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China,Guangxi Key Laboratory of Maine Environmental Science, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;College of Light Industry and Food Engineering, Guangxi University, Nanning, Guangxi, 530004, China,Guangxi Key Laboratory of Maine Environmental Science, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;College of Light Industry and Food Engineering, Guangxi University, Nanning, Guangxi, 530004, China and College of Light Industry and Food Engineering, Guangxi University, Nanning, Guangxi, 530004, China
Abstract:Objective] To investigate the chemical constituents of the fermentation liquid from marine gorgonian-associated bacterium Bucillus methylotrophicus.Method] The compounds were isolated and purified by column chromatography on silica gel and their structures were identified by spectral analyses and comparison with literatures.Result] Six compounds were obtained from the fermentation liquid of B.methylotrophicus and characterized as Cyolo-(Pro-Gly) (1), Cyolo-(S-pro-R-leu) (2), Thymidine (3), Uridine (4), Protocatechuic acid (5), 3-Furancarboxylic acid-5-(hydroxmenthy)-Furan (6).Conclusion] Compounds 1~6 were isolated from B.methylotrophicus for the first time, which enriches the chemical diversity of marine gorgonian-associated bacterium Bucillus methylotrophicus.
Keywords:gorgonian  marine bacteria  compouds  chemical identification
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