两株氧化葡萄糖酸杆菌静息细胞的催化性能比较

【目的】氧化葡萄糖酸杆菌是一种严格好氧的革兰氏阴性菌,能够立体选择性氧化多种羟基化合物生成相应的醛、酮或酸。【方法】本文将比较两株氧化葡萄糖酸杆菌DSM 2003和621H的静息细胞对甘油、乙二醇及乙醇的催化能力,并研究了溶氧对催化反应的影响。【结果】两株氧化葡萄糖酸杆菌氧化甘油的能力较相似,溶氧对这两株菌的甘油催化能力都有较大影响;氧化葡萄糖酸杆菌DSM 2003能够将乙二醇几乎全部氧化为羟基乙酸,副产物少,而621H转化相同量的底物只生成较少量的羟基乙酸,副产物多。氧化葡萄糖酸杆菌621H生产乙酸的能力也很低。【结论】两株氧化葡萄糖酸杆菌催化甘油生成二羟基丙酮的能力相当,但在催化醇生成酸的反应中,氧化葡萄糖酸杆菌DSM 2003更具优势。

Comparison of the catalytic property by the resting cells of two Gluconobacter oxydans strains

Objective] Gluconobacter oxydans is a strictly aerobic gram-negative bacterium that can stereo selectively oxidize a variety of hydroxy compounds to produce the corresponding aldehydes, ketones or acids.Methods] The purpose of this study was to compare the oxidation ability of glycerol, glycol and ethanol by resting cells of G.oxydans DSM 2003 and 621H, and the influence of dissolved oxygen on catalytic reaction.Results] Two strains of oxidized G.oxydans have similar ability to oxidize glycerol.Dissolved oxygen has a great influence on the glycerol catalytic ability of the two strains.G.oxydans DSM 2003 can almost completely oxidize ethylene glycol to glycolic acid with few by-products.While 621H transformed the same amount of substrate, only a smaller amount of glycolic acid was produced, with many by-products.Oxidized G.oxydans 621H also has low acetic acid production.Conclusion] The two strains of oxidized G.oxydans have similar ability to oxidate glycerol to dihydroxyacetone, whereas, G.oxydans DSM 2003 shows more advantages in the reactions of alcohols to generate acids.