蔗渣木聚糖制备低聚木糖的最优复合酶降解工艺研究

【目的】研究复合酶酶解蔗渣木聚糖制备低聚木糖的方法。【方法】采用混料试验设计中的单纯形质心方法对木聚糖酶 A，木聚糖酶B和α-L-阿拉伯糖苷酶3种酶进行配方设计试验，以低聚木糖得率为评价指标。【结果】低聚木糖得率的最优酶复合配比为木聚糖酶 A 39．5％，木聚糖酶B 25％，α-L-阿拉伯糖苷酶35．5％，在此条件下预测低聚木糖得率为85．20％。【结论】该配比能显著提高蔗渣木聚糖酶解效率和低聚木糖的产率。

The Research on Optimal Production Process of Xylooligosaccharides by Composite Enzymatic Hydrolysis of Bagasse Xylan

Objective]The production of xylooligosaccharides was studied by composite enzymat-ic hydrolysis of bagasse xylan.Methods]The formulation of xylanase A,xylanase B and alpha-L-arabinofuranosidase was opitimized through simplex-centroid method for mixture design ex-periments,with the XOS extraction rate as evaluation index.Results]The best formula was de-termined as：Xylanase A 39.5%,xylanase B 25% and alpha-L-arabinofuranosidase 35.5%.Un-der these conditions,the extraction rate of XOS was 85.20%.Conclusion]The optimization rate of compound enzyme has the characteristic of increasing yield and efficiency.