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| 利用抗药性选育盐霉素高产菌株 | |
| 引用本文： | 吴健,戴桂馥,李宗伟,张晓红,霍裕平.利用抗药性选育盐霉素高产菌株[J].郑州大学学报(理学版),2005,37(4):92-95. |
| 作者姓名： | 吴健 戴桂馥 李宗伟 张晓红 霍裕平 |
| 作者单位： | 1. 郑州大学生物工程系,郑州,450001 2. 郑州大学物理工程学院,郑州,450001 3. 泓天威药业有限公司,河南,濮阳,457100 |
| 摘 要： | 采用紫外线诱变和紫外线复合氯化锂处理盐霉素生产菌株,利用含棕榈油的筛选平板,结合选育脂肪酶活力高的菌株,成功获得产量提高并适应棕榈油发酵的高产菌株Ae-4.棕榈油完全替代豆油摇瓶113 h发酵的产量是豆油发酵的88.7%(出发株为69.6%).进一步通过选育抗药性突变株获得了抗链霉素的高产突变株E4Lt-1(摇瓶发酵产量提高57.4%)和抗利福霉素的高产突变株Rift-1-2(摇瓶发酵产量提高44.9%). |
| 关 键 词： | 白色链霉菌 盐霉素 紫外线诱变 抗药性 |
| 文章编号： | 1671-6841(2005)04-0092-04 |
| 收稿时间： | 12 22 2004 12:00AM |
| 修稿时间： | 2004年12月22 |
The Breeding of High Yield Salinomycin Strains of Streptomyces albus Through Antibiotic-resistant Strains Screening |
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| Wu Jian,Dai Guifu,Li Zongwei,Zhang Xiaohong,Huo Yuping.The Breeding of High Yield Salinomycin Strains of Streptomyces albus Through Antibiotic-resistant Strains Screening[J].Journal of Zhengzhou University:Natural Science Edition,2005,37(4):92-95. | |
| Authors: | Wu Jian Dai Guifu Li Zongwei Zhang Xiaohong Huo Yuping |
| Abstract: | A Streptomyces albus strain for industrial production of salinomycin is successfully mutated by UV radiation and screened on plates containing palm oil.The mutant Ae-4 which could produce high yield salinomycin using palm oil as carbon source is obtained according to its high lipase activity on the screening plate.By substituting palm oil for bean oil completely,the salinomycin yield of Ae-4 in shake flask is 88.7% of that fermented with bean oil,where as for the original strain,the value is only 69.6%.Further more E4Lt-1,a streptomycin -resistant mutant with enhanced(57.4% higher) salinomycin production and Rift-1-2,a rifampin-resistant mutant with enhanced(44.9% higher) salinomycin production are screened from Ae-4 by UV radiation. |
| Keywords: | Streptomyces albus salinomycin UV radiation drug resistance |
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