| 灰玫瑰青霉产生的抗菌物质AF1的分离与活性研究 |
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| 引用本文： | 杨炜华,,卢雪梅,张为灿,高培基.灰玫瑰青霉产生的抗菌物质AF1的分离与活性研究[J].山东大学学报(理学版),2009,44(7):1-4. |
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| 作者姓名： | 杨炜华 卢雪梅 张为灿 高培基 |
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| 作者单位： | 1. 山东大学微生物技术国家重点实验室, 山东 济南 250100; 2. 山东省医学科学院基础医学研究所, 山东省现代医药与技术重点实验室, 山东 济南 250062 |
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| 基金项目： | 国家自然科学基金资助项目(30600026);山东省优秀中青年科学家科研奖
励基金资助项目(2007BS03002) |
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| 摘 要： | 从灰玫瑰青霉Penicillium griseoroseum发酵液中提取到1种具有抗菌活性的物质AF1,对其性质进行初步研究。发酵液经纱布过滤,离心后经5?ku超滤膜超滤,经Sephadex LH 20和HPLC分离纯化得到纯净物质AF1,并对其生物学活性进行研究。结果证明:AF1对β 内酰胺酶和酸碱稳定,对热有较好的耐受性,不是青霉素类抗菌物质;相对分子质量约为1?465,结构中含有糖和氨基特征,对金黄色葡萄球菌的MIC为80?μg／mL,对抗药金黄色葡萄球菌有效且不容易诱导细菌抗药性产生。
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| 关 键 词： | 青霉 抗菌 分离纯化 生物活性 |
| 收稿时间： | 2009-02-05 |
Isolation and characterization of antibacterial factor AF1
produced by Penicillium griseoroseum |
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| Institution: | 1. State Key Laboratory of Microbial Technology, Shandong University , Jinan 2501 00, Shandong, China； 2. Key Laboratory for Modern Medicine and Technology of Shan dong Province, the Institute of Basic Medicine, Shandong Academic of Medicine Science, Jinan 250062, Shandong, China |
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| Abstract: | An antimicrobial factor termed AF1 was isolated from culture broth of Penicillium griseoroseum. The filtered fermentation broth underwent centrifugation and ultrafiltration (membrane cutoff 5?kD). Then it was purified using Sephadex LH 20 and high performance liquid chromatography (HPLC). The molecular weight of AF1 was 1465 Da. AF1 was stable to β lactamase, hydroxylamine, acid, alkali and heat. Together, these data indicate that AF1 is a novel antibacterial factor which is different from penicillin. The molecular structure of AF1 shows characteristics of saccharide and amid. MIC of AF1 versus Staphylococcus aureus is 80?μg／ml, and AF1 is effective to drug resistant S. aureus. It is difficult to induce bacterial resistant to AF1. |
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| Keywords: | Penicillium antibiotic isolation characterization |
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