| 中国对虾抗菌肽成熟肽的cDNA克隆 |
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| 引用本文： | 康翠洁,王金星,赵小凡,相建海.中国对虾抗菌肽成熟肽的cDNA克隆[J].山东大学学报(理学版),2002,37(6):552-556,565. |
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| 作者姓名： | 康翠洁 王金星 赵小凡 相建海 |
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| 作者单位： | 1. 山东大学生命科学学院,济南,250100
2. 中国科学院海洋研究所,青岛,266071 |
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| 基金项目： | 国家重大基础研究 ( 973)项目G19990 12 0 0 7，国家高技术研究发展计划 ( 86 3)项目 2 0 0 1AA6 2 112 0 ,2 0 0 2AA6 2 90 9资助 |
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| 摘 要： | 利用RT－PCR、嵌套PCR（Nested PCR)和3’－RACE等方法，从中国对虾血细胞中克隆到1种抗菌肽基因片段，称为中国对虾肽（Chp）基因。此基因片段长543bp，开读框共有156个碱基，编码52个氨基酸。该基因所编码的氨基酸序列对应于凡那对虾抗菌肽成熟肽的序列，与其一致性为52－59％，相似性为61－73％，分子量为5652．4Da，理论等电点为9．76，带正点荷的氨基酸（Arg-Lys）为8个，不含带负电荷的氨基酸。上述这些特征（分子量较小，带正点荷）均为抗菌肽的普遍特征。
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| 关 键 词： | 抗菌肽 cDNA克隆 嵌套PCR 3‘－RACE 中国对虾 |
| 文章编号： | 1671-9352(2002)06-0552-05 |
Molecular Cloning of the Cdna Encoding a Mature Antimicrobial Peptide from Shrimp, Penaeus Chinensis |
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| KANG Cui-jie ,WANG Jin-xing ,ZHAO Xiao-fan & XIANG Jian-hai.Molecular Cloning of the Cdna Encoding a Mature Antimicrobial Peptide from Shrimp, Penaeus Chinensis[J].Journal of Shandong University,2002,37(6):552-556,565. |
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| Authors: | KANG Cui-jie WANG Jin-xing ZHAO Xiao-fan & XIANG Jian-hai |
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| Institution: | KANG Cui-jie 1,WANG Jin-xing 1,ZHAO Xiao-fan 1& XIANG Jian-hai 2 |
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| Abstract: | We have cloned a new gene fragment of an antimicrobial peptide,mature ch-penaeidin gene,from the hemocyte cDNAs of the Chinese shrimp,Penaeus chinensis,by RT-PCR,Nested PCR and 3'-RACE methods.The nucleotide sequence of the fragment,ch-penaeidin,consists of 543bp,and has open reading frame of 156bp.Encoding 52 amino acid residues.The deduced amino acid sequence of putative showed 52-59% amino acide sequence identity with that of penaeidin from Litopenaus vannamei,lv-penaeidins.Sequence comparison showed that ch-penaeidin is similar to lv-penaeidins 3C(73%).The molecular mass of the ch-penaeidin is 5652.4Da,theoretical pI 9.76.It has 8 positively charged residues(Arg+Lys),and no negatively charged one. |
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| Keywords: | antimicrobial peptide cDNA clone Nested PCR 3'-RACE Penaeus chinensis |
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