大麦黄矮病毒（BYDV）外壳蛋白基因在小麦原生质体中的稳定转化

实验采用ＰＥＧ介导法转化小麦，用ＧＵＳ基因作为标志，用荧光法测定Ａｃｔｌ－ＧＵＳ、Ｅｍｕ－ＧＵＳ、３５Ｓ－ＧＵＳ三种质粒转化小麦细胞后的瞬间表达强度，以比较Ａｃｔｌ、Ｅｍｕ、３５Ｓ三种启动子在小表中的表达强度．结果表明，Ａｃｔｌ和Ｅｍｕ的强度大致相等，均比３５Ｓ强．采用Ｅｍｕ为启动子带ＢＹＤＶＣＰ基因的质粒和经改造过的Ａｃｔ１为启动子带有抗潮霉素选择标记基因的质粒共转化小麦“济南１７７”的原生质体．转化６ｄ后，用潮霉素进行筛选，最后得到两块抗潮霉素的愈伤组织，转化后４个月，经ＰＣＲ检测，证明ＣＰ基因已整合进一块愈伤组织的细胞基因组中．

THE STABLE TRANSFORMATION OF THE COAT PROTEIN(CP) GENE OF BARLEY YELLOW DWARF VIRUS(BYDV) IN PROTOPLASTS OF WHEAT

The protoplast was used as a transforming system to transfer the coat protein(CP) gene of the Barlay Yellow Dwarf Virus (BYDV) into wheat by the PEG method. The activity of three promoters for transient experssion have been compared with GUS gene as the marker. Rice Actl promoter and recombined maize Emu promoter are more efficient than CaMV 355 promoter in wheat. The Emu-CP plasmid and Actl-Hpt (Hygromycin Phosphatase)plasmid were used to cotransform the protoplasts of the wheat cv. Jinanl77 which were then selected by hygromycin B(Hm). Afterwards. two calli were obtained. After transformation for 4 months, they were analayzed for the presence of CP gene by PCR method and one of them was positive. The differentiation of the transformed callus was in progress.