束骨姜黄化学成分及其乙酰胆碱酯酶抑制活性

采用硅胶层析、反相硅胶中压柱层析、高效液相等色谱技术对姜科姜黄属束骨姜黄(Curcuma xanthorrhiza Roxb.)根茎中的低极性化学成分进行了分离纯化,并通过MS和NMR等波谱技术确定了化合物结构,分离纯化化合物6个,分别鉴定为蓬莪术环氧酮(Ⅰ)、β 谷甾醇(Ⅱ)、愈创木内酯Guai-1(10),3,5,7(11),8-pentaen-2-on-11,8-olide(Ⅲ)、郁金二酮(Ⅳ)、莪术双环烯酮(Ⅴ)、花侧柏烯(Ⅵ)。化合物Ⅲ、Ⅵ为首次从该科植物中分离得到,化合物Ⅳ、Ⅴ为首次从该种中分离得到。对所分离获得化合物进行了乙酰胆碱酯酶抑制活性测定,其中化合物Ⅳ、Ⅵ具有活性,化合物Ⅳ的TLC生物自显影最小抑制量为95μg。

Chemical constituents of Curcuma xanthorrhiza Roxb.and their acetylcholinesterase inhibitory activity

Chemical constituents from Curcuma xanthorrhiza Roxb. (Zingiberaceae) rhizome were isolated and purified by column chromatography (silica gel, RP silica gel MPLC, HPLC) and their structures were elucidated on the basis of spectral analyses. Six compounds were identified by MS and NMR spectroscopy as zederone(Ⅰ), β sitosterol(Ⅱ), Guai-1(10),3,5,7(11),8-pentaen-2-on-11,8-olide(Ⅲ), Curcumadione(Ⅳ), Curcumenone (Ⅴ), Cuparenol (Ⅵ). Compound Ⅲ and Ⅵ were isolated for the first time from the plants of Zingiberaceae, and compound Ⅳand Ⅴ were isolated from Curcuma xanthorrhiza Roxb. for the first time. Meanwhile, the acetylcholinesterase inhibitory activity of the isolated compounds was evaluated. Compounds Ⅳ and Ⅵ showed certain inhibitory activity, the minimum inhibitory quantity of Ⅳ on TLC bioautography test was 95μg.