| 两种中草药的SOD活性测定及其对细胞体外培养存活时间的影响 |
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| 引用本文： | 邹岳奇,刘玉翠,陈英珠,张继全,陈幼春,陈韶华.两种中草药的SOD活性测定及其对细胞体外培养存活时间的影响[J].河北省科学院学报,1994(4). |
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| 作者姓名： | 邹岳奇 刘玉翠 陈英珠 张继全 陈幼春 陈韶华 |
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| 作者单位： | 河北省科学院生物研究所，中国农业科学院畜牧研究所 |
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| 摘 要： | 分别以改良的连苯二酚自氧化法和极谱氧电极法测量了两种药用植物（祁州漏芦和女贞子）提取液ＭＰＥ－１和ＭＰＥ－２含有的超氧化物歧化酶（ＳＯＤ）活性。检测结果表明，ＭＰＥ－１和ＭＰＥ－２的ＳＯＤ活性分别为１３１．３ｕ／ｍｌ和２９８．５ｕ／ｍｌ．以ＭＰＥ－１和ＭＰＥ－２为培养液添加物的人红细胞体外培养试验证实，这两种中药提取液均能稍微延长人红细胞体外培养的存活时间。
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| 关 键 词： | 祁州漏芦（Rhaponticum nuiflorum L．DC），女贞子（Ligustrum lucidum Ait），超氧化物歧化酶（Superoxide dismutase），人红细胞（Human erythrocyte），细胞体外培养（In vitro） |
DETECTION OF SUPEROXIDE DISMUTASE(SOD) ACTIVITY IN TWO MDEICINAL PLANTS AND THEIR EFFECTS ON LIVING TIME OF ERYTHROCYTE IN VITRO |
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| Zou Yueqi,Liu Yucui,Chen Yingzhu,Zhang Jiqian, Chen Yiuchun,Chen Shaohua.DETECTION OF SUPEROXIDE DISMUTASE(SOD) ACTIVITY IN TWO MDEICINAL PLANTS AND THEIR EFFECTS ON LIVING TIME OF ERYTHROCYTE IN VITRO[J].Journal of The Hebei Academy of Sciences,1994(4). |
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| Authors: | Zou Yueqi Liu Yucui Chen Yingzhu Zhang Jiqian Chen Yiuchun Chen Shaohua |
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| Institution: | Sience Philosophy Betterment Society In |
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| Abstract: | The superoxied dismutase(SOD)activity Of two medicinal plant extracts(MPE-1 and MPE-2),extracted from Rhaponticum uniflorum DC. and Ligustrum lucidum Ait,respectively,Was detected with modified Pyrogallol autokidation method and the polarographic oxygen electrode method.The results revealed that the SOD activity of MPE-2 was higher than that of MPE-1,and they were 298. 5 u/nd and 131.3u/ml respectively. The experiment of human erythrocyte culture in vitro with MPE-1 and MPE-2 as additives was completed,and the result showed that both the two extracts prolonged slightly the living time of human erythrocytes in Vitro. |
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| Keywords: | Rhapontieum uniflorum L DC Ligustrum lucidum Ait Superoalde dismutase Human erythrocyte In vitro |
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