| 芥菜原生质体培养和胚性愈伤组织的产生 |
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| 引用本文： | 赵军良
,张江涛
,陈柔如
,周晓梅
,刘少翔.芥菜原生质体培养和胚性愈伤组织的产生[J].山西大学学报(自然科学版),1989(2). |
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| 作者姓名： | 赵军良 张江涛 陈柔如 周晓梅 刘少翔 |
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| 基金项目： | 山西省自然科学基金~~ |
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| 摘 要： | 以芥莱无菌苗下胚轴为材料游离获得原生质体,原生质体培养于含NAA1.5mg/l,6-BA0.6mg/l、和2.4-D0.5mg/l的DPD液体培养基中,约4—5周形成肉眼可见的小愈伤组织。转入MS固体培养基后,即可产生大量的愈伤组织,其中胚性愈伤组织约占50%以上。
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| 关 键 词： | 芥菜 原生质体 胚性愈伤组织 |
CULTURE OF PROTOPLASTS AND FORMATION OF EMBRYOGENETIC CALLI FROM Brassica juncea (L.) Czern. et Coss. |
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| Zhao Junliang Zhang Jiangtao Chert Rouru Zhou Xiaomei Liu Shaoxiang.CULTURE OF PROTOPLASTS AND FORMATION OF EMBRYOGENETIC CALLI FROM Brassica juncea (L.) Czern. et Coss.[J].Journal of Shanxi University (Natural Science Edition),1989(2). |
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| Authors: | Zhao Junliang Zhang Jiangtao Chert Rouru Zhou Xiaomei Liu Shaoxiang |
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| Institution: | Zhao Junliang Zhang Jiangtao Chert Rouru Zhou Xiaomei Liu Shaoxiang |
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| Abstract: | Hypocotyl protoplasts from Brassica juncea (L.) Czern. et Coss were cultured in DPD liquid medium containing NAA 1.5rag/1, 6-BA 0.6rag/1, and 2.4-D 0.5mg/1.Protoplasts form microcolonies in 4-5 weeks and develop into a number of calli after being transfered to MS solid medium. About fifty percent of total calli are embryogenetic calli. |
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| Keywords: | Brassiea juncea protoplasts embryogenetic calli |
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