沼泽红假单胞菌CQV97菌株对污染水体三氮去除特性研究

在不同污染程度模拟水体中,利用沼泽红假单胞菌CQV97,在厌氧光照条件下,研究了水体中氨氮、硝态氮和亚硝态氮含量、菌体生物量和水体pH的变化关系.随时间延长,CQV97菌株对氨氮、硝态氮或亚硝态氮去除量增大,生物量增加,水体pH升高;随氨氮浓度提高,生物量增加,氨氮低于33.2mg/L能被完全去除,最大去除量达84.2mg/L,水体pH维持在9.2～9.4;随硝态氮浓度的升高,菌体生物量降低,浓度低于216.96mg/L能被完全去除,pH维持在9.1～9.3.随亚硝态氮浓度增加,菌体生长延滞期延长,生物量和pH升高幅度降低,浓度低于128.2mg/L能被完全去除.结果表明,CQV97菌株对氨氮、硝氮和亚硝氮具有良好的去除能力.

Denitrifying Characterizations from Inorganic Nitrogen-polluted Wastewater by Rhodopseudomonas palustris CQV97

The relationship among removal amount and removal rate of ammonia nitrogen,nitrate nitrogen and nitrite nitrogen,biomass as well as pH change in simulation wastewater polluted by different nitrogen compounds with Rhodopseudomonas palustris CQV97 were investigated under anaerobically in the light.The results showed that strain CQV 97 had better capability of ammonia nitrogen removal.The removal amount and removal rate of ammonia nitrogen,biomass and pH increased during 10 d.With the increasing ammonia nitrogen concentration,the removal amount of ammonia nitrogen and biomass enhanced,pH reached at 9.2～9.4,but the apparent removal rate of ammonia nitrogen reduced.Less than 33.2 mg/L ammonia nitrogen could be completely removed.The maximal removal amount of ammonia nitrogen reached 84.2 mg/L.In the nitrate nitrogen-containing polluted wastewater,strain CQV97 could utilize,transform and rapidly and effectively remove nitrate nitrogen,cell growth and pH gradually increased while the nitrate nitrogen was constantly removed.With increasing nitrate nitrogen concentration,the biomass decreased,pH maintained at 9.1～9.3.The nitrate nitrogen could be removed completely and the maximal removal amount of nitrate nitrogen reached 216.96 mg/L.Strain CQV97 also was able to utilize and transform nitrite nitrogen in wastewater polluted by nitrite nitrogen,but cell growth showed lag phase,pH rised no more than 7.5.With increasing nitrite nitrogen concentration,the increasing rate of cell growth and pH reduced.The removal rate of nitrite nitrogen could reach 100% when the amount of nitrite nitrogen below 128.2 mg/L.In general,strain CQV97 has better removal ability to ammonia nitrogen,nitrate nitrogen and nitrite nitrogen.