| 棒束孢霉胞外多糖的纯化 |
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| 引用本文： | 褚西宁,程巧梅.棒束孢霉胞外多糖的纯化[J].山西大学学报(自然科学版),1996,19(3):315-318. |
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| 作者姓名： | 褚西宁 程巧梅 |
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| 作者单位： | 山西大学生命科学系 |
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| 基金项目： | 山西省自然科学基金资助项目 |
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| 摘 要： | 棒束孢霉发酵上清液经减压浓缩、醇析、蛋白酶法与Ｓｅｖａｇ法结合脱蛋白、逆向流水透析和ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ－５０柱层析等步骤，获得了胞外纯多糖。用ＴＬＣ、ＧＣ及ＩＲ分析表明，该多糖链由Ｄ－甘露醇和Ｄ－半乳糖组成。
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| 关 键 词： | 棒束孢霉，真菌多糖，纯化 |
PURIFICATION OF THE EXTRACELLULAR POLYSACCHARIDES CULTURED FROM ISARIA SP. |
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| Chu Xining,Chen Quamei,Yuan Jingming.PURIFICATION OF THE EXTRACELLULAR POLYSACCHARIDES CULTURED FROM ISARIA SP.[J].Journal of Shanxi University (Natural Science Edition),1996,19(3):315-318. |
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| Authors: | Chu Xining Chen Quamei Yuan Jingming |
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| Abstract: | A strain named Isaria sp. was separated and purified from Cordyceps sinensis.The extracellular polysaccharide from 7 days culture of the strain were purified by some steps,eg.concentration in vacum,preciptation with alcohol,deprotrinization with both subtilism and sevag procedure as well as chromatography on DEAE-Sephadex A-50 column.The results from TLC,GC and IR analysis showed that the polysaccharide consisted of two compounds:D-galactose and D-mannose only. |
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| Keywords: | Isaria sp polysaccharide purification |
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