| 几种草坪草组织培养和植株再生的研究 |
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| 引用本文： | 王慧,许瑾,周小梅,韩玉杰,张彦芹.几种草坪草组织培养和植株再生的研究[J].山西大学学报(自然科学版),2009,32(3). |
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| 作者姓名： | 王慧 许瑾 周小梅 韩玉杰 张彦芹 |
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| 作者单位： | 1. 山西大学生命科学与技术学院,山西,太原,030006
2. 山西省农业科学院旱地农业中心,山西,太原,030031 |
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| 摘 要： | 以6种草坪草种子和2种草坪草愈伤组织为材料,研究了基本培养基种类和2,4-D浓度对愈伤组织诱导以及不同的细胞分裂素对分化的影响.试验结果表明,N6培养基较MS、NB培养基适合草坪草组织培养,诱导愈伤的2,4-D浓度宜采用6 mg/L,有机添加物对愈伤组织的生长有利,特别是肌醇对草坪草愈伤组织的生长有明显的促进作用.在分化培养中,不同材料所需的细胞分裂素适宜种类需要通过试验来确定.
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| 关 键 词： | 草坪草 组织培养 愈伤组织 有机添加物 |
Study on the Tissue Culture and Plant Regeneration of Turfgrasses |
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| WANG Hui,XU Jin,ZHOU Xiao-mei,HAN Yu-jie,ZHANG Yan-qin.Study on the Tissue Culture and Plant Regeneration of Turfgrasses[J].Journal of Shanxi University (Natural Science Edition),2009,32(3). |
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| Authors: | WANG Hui XU Jin ZHOU Xiao-mei HAN Yu-jie ZHANG Yan-qin |
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| Abstract: | The effects of some media and concentration of 2,4-D on callus-induction with 6 kinds of Turf-grass seeds and 2 kinds of Turfgrass calli,and 3 sorts of cytokinins on callus differentiation were studied. The results showed that N_6 is the optimal medium in all of the three media tested and the proper concentration of 2,4-D is 6 mg/L. The organic supplements used in subculture media, especially inositol, benefited callus growth. In differentiation culture,the suitable kinds of cytokinins needed for different materials must be decided by trial. |
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| Keywords: | 2 4-D |
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