| 华北落叶松多酚氧化酶动力学特性及其同工酶的研究 |
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| 引用本文： | 张淑改,姚延.华北落叶松多酚氧化酶动力学特性及其同工酶的研究[J].山西大学学报(自然科学版),1999,22(1):61-64. |
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| 作者姓名： | 张淑改 姚延 |
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| 作者单位： | 山西农业大学林学系,太谷,030801 |
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| 摘 要： | 文中对华北落叶松根系多酚氧化酶动力学特性及其同工酶进行分析。结果表明：该酶对邻苯二酚（二元酚）和焦性没食子酸（三元酚）都能氧化，但对前者亲和力较强；ｐＨ７．０时酶活性最强；Ｃｕ２＋提高多酚氧化酶活性；ＥＤＴＡ，Ｈ３ＢＯ３及半胱氨酸可抑制该酶活性；聚丙烯酰胺凝胶电泳将根系多酚氧化酶分出７条同工酶带。
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| 关 键 词： | 华北落叶松，多酚氧化酶，动力学特性，同工酶 |
Research of Polyphenoloxidase Kinetic Properties and Iso zymes of Larix principis-rupprechtii |
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| Zhang Shugai,Yao Yantao,Chang Mingchang,Xue Junjie.Research of Polyphenoloxidase Kinetic Properties and Iso zymes of Larix principis-rupprechtii[J].Journal of Shanxi University (Natural Science Edition),1999,22(1):61-64. |
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| Authors: | Zhang Shugai Yao Yantao Chang Mingchang Xue Junjie |
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| Abstract: | This paper studies polyphenoloxidase kinetic properties and isozymes from roots of Larix principis-rupprchtii. Results show that both catechol and 1.2.3-hydroxyphenyl can oxidaze polyphenoloxidase.But the second has a higher affinity to polyphenoloxidase.When pH is 7.0,polyphenoloxidase has the biggest activity.Cu 2 can increase its activity.Polyphenoloxidase of roots was analysed by PAGE,approximately seven bands were detected. |
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| Keywords: | kinetic property isozyme polyphenoloxidase Classification Code of Chinese Literatures O945 |
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