青霉DNA提取方法比较研究

比较了CTAB、SDS-CTAB和氯化苄等3种DNA提取方法提取青霉DNA的效果，结果表明：CTAB法和SDS-CTAB法只适合于部分青霉菌株的DNA提取，另外一些菌株则提取不出谱带清晰的DNA．而氯化苄法适合于供试的所有青霉菌株的DNA提取，所有菌株的DNA凝胶电泳图谱带清晰，效果很好．此外，还研究了液氮研磨和提取液pH对氯化苄法提取DNA效果的影响．

Comparision of Methods of Extracting DNA from Penicillium

Three kinds of methods(i.e.benzyl choeide method, CTAB method and SDS-CTAB method) were used to extract DNA from ten strains of Penicillium.The results showed that the benzyl choeide method was able to be used to extract the DNA of those Penicillium strains, and bands resulting from gel electrophoretic separations were very clear. However, both the CTAB and SDS-CTAB methods were only adequate to be used to extract DNA of some strains of Pencicillium, and the DNA bands of some strains were clear, but others were not obvious. The effects of both the pH values of extracts and the grinding of mycelia in liquid nitrogen on the result of DNA extracting were also discussed.