| 人可溶性TRAIL原核分泌表达载体的构建 |
| |
| 引用本文： | 杨峰丽,梁雅丽,赵良启,赵邑.人可溶性TRAIL原核分泌表达载体的构建[J].山西大学学报(自然科学版),2011(Z2):116-119. |
| |
| 作者姓名： | 杨峰丽 梁雅丽 赵良启 赵邑 |
| |
| 作者单位： | 山西大学生物技术研究所;山西省生物研究所基因药物研究室; |
| |
| 摘 要： | 为了克隆人可溶性TRAIL基因片段,构建其新型原核分泌表达载体,从HL-60细胞中提取总RNA,根据GeneBank提供的人TRAIL基因序列,设计扩增人可溶性TRAIL基因114～281片段的特异性引物,同时引入NcoI、BamHI、TEV酶的酶切位点及His标签,以便纯化及纯化后切去His标签,并将目的基因克隆至原核表达载体PhoA,经测序分析鉴定,于大肠杆菌MM294中进行表达.结果表明:克隆到人sTRAIL基因序列,经DNA测序结果与GeneBank基因库报道的一致,成功构建了可分泌表达的人源可溶性TRAIL原核表达载体PhoA-sTRAIL,并在大肠杆菌MM294中成功表达.
|
| 关 键 词： | 可溶性TRAIL 克隆 原核分泌表达 |
Construction of Prokaryotic Secretory Expression Vector of Human Soluble TRAIL |
| |
| YANG Feng-li,LIANG Ya-li,ZHAO Liang-qi,ZHAO Yi.Construction of Prokaryotic Secretory Expression Vector of Human Soluble TRAIL[J].Journal of Shanxi University (Natural Science Edition),2011(Z2):116-119. |
| |
| Authors: | YANG Feng-li LIANG Ya-li ZHAO Liang-qi ZHAO Yi |
| |
| Institution: | YANG Feng-li1,LIANG Ya-li2,ZHAO Liang-qi1,ZHAO Yi2(1.Institute of Biotechnology,Shanxi University,Taiyuan 030006,China,2.Gene-based drug lab.Biology Institute of Shanxi,China) |
| |
| Abstract: | In order to explore cloning of human soluble TRAIL gene fragments to construct its new prokaryotic secretion expression vector,from HL-60 cells to extract total RNA,according to GeneBank provide human TRAIL gene sequence,amplification of human soluble TRAIL gene fragments of 114 to 281 primers,and the introduction of NcoI,BamHI,TEV enzyme restriction site and His tag for purification and purified truncated His tag,and the purpose of gene cloning to prokaryotic expression vector PhoA,identified by sequence a... |
| |
| Keywords: | sTRAIL cloning prokaryotic secretory expression |
| 本文献已被 CNKI 等数据库收录！ |
|
