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| 用RAPD技术分析烟草与曼陀罗体细胞杂种 | |
| 引用本文： | 李君剑,周小梅,郭双生,李文彬,梁爱华.用RAPD技术分析烟草与曼陀罗体细胞杂种[J].山西大学学报(自然科学版),2004,27(2):195-198. |
| 作者姓名： | 李君剑 周小梅 郭双生 李文彬 梁爱华 |
| 作者单位： | 1. 山西大学,生命科学与技术学院,山西,太原,030006 2. 航天医学工程研究所,北京,100094 3. 中国科学院遗传研究所,北京,100101 |
| 摘 要： | 利用RAPD技术对普通烟草(Nicotiana tabacum L.cv Xanthi nc)和毛曼陀罗(Datura innoxia Mill)原生质体融合获得的体细胞杂种进行了分析.使用了9个10-mer随机引物对7个体细胞杂种和双亲进行RAPD指纹图谱分析,从7个杂种的9组DNA指纹图谱上共得到570条扩增带,其中与烟草特征谱带相同的带数占了67.8%,与双亲共有的谱带相同的带数为24.6%,而与曼陀罗特征谱带相同的带数仅有2.8%.由此可见,杂种组织倾向于排除毛曼陀罗的DNA,融合体是一个高度不对称的体细胞杂种.此外,杂种中还出现了双亲没有的谱带(新带占4.8%). |
| 关 键 词： | 烟草 毛曼陀罗 体细胞杂种 |
| 文章编号： | 0253-2395(2004)02-0195-04 |
| 修稿时间： | 2003年9月2日 |
RAPD Analysis of Somatic Hybrids between Tobacco and Hairy Datura |
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| LI Junjian,ZHOU Xiaomei,GUO Shuangsheng,LI Wenbin LIANG Aihua.RAPD Analysis of Somatic Hybrids between Tobacco and Hairy Datura[J].Journal of Shanxi University (Natural Science Edition),2004,27(2):195-198. | |
| Authors: | LI Junjian ZHOU Xiaomei GUO Shuangsheng LI Wenbin LIANG Aihua |
| Institution: | LI Junjian~1,ZHOU Xiaomei~1,GUO Shuangsheng~2,LI Wenbin~3 LIANG Aihua~1 |
| Abstract: | The somatic hybrids between tobacco(Nicotiana tabacum L.cv Xanthi nc)and hairy datura(Datura innoxia Mill.)were obtained by PEG/DMSO fusion method.The somatic hybrids were identified with RAPD.Among a total of 570 bands observed from 7 somatic hybrid plants after DNAamplification with 9 arbitrary 10-mer primers,386(67.8 %) were tobacco-specific bands and 141(24.6 %)showed common bends of both the parents,only 16(2.8 %) homologue to hairy datura-specific bands.The results suggested that besides containing nuclear substances of two parents,the hybrid genomes were inclined to eliminate hairy datura chromosomes with DNA reconstruction.The hybrids were asymmetric.There were 4.8 % new bands(not seen in two parents)shown in the RAPD fingerprints of somatic hybrids. |
| Keywords: | RAPD |
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