HPLC法测定银杏叶提取物中总黄酮醇苷的含量

目的：建立测定银杏叶提取物中总黄酮醇苷的含量测定方法.方法：用HPLC方法对测定银杏叶提取物中总黄酮醇苷进行含量测定研究.色谱柱采用Diamonsil C18（200×4.6 mm 5μm）,以甲醇-0.4%磷酸溶液（52∶48）为流动相,检测波长为360 nm;流速为1.0 mL/min.结果：槲皮素在0.174-0.870μg线性关系良好（r=0.999 8,n=5）,山柰素在0.117-0.585μg线性关系良好（r=0.999 8,n=5）,异鼠李素在0.040 8-0.204μg线性关系良好（r=0.999 9,n=5）;槲皮素、山柰素、异鼠李素的平均回收率（n=5）分别为99.96%、99.24%、100.46%.结论：本方法操作简便,重现性好,专属性强,可作银杏叶提取物中总黄酮醇苷的质量控制方法.

HPLC Determination of Total Flavonoids in the Ginkgo Biloba Extract

Objective：To establish an HPLC method for determination of flavones in the Ginkgo Biloba extract. Method： The chromatographic column was Diamonsil C18 （200 × 4.6 mm, 5 μm）, The mobile phase consisted of 0.5% phosphate buffer/methanol（48 ： 52）, the flow rate was 1.0 mL/min, UV detection wavelength was 360 nm. Results ： According to the selected chromatographic conditions, the linear ranges of quercetin, kaempferide and isorhamnetin were 0. 174 - 0. 870 μg, 0.117 - 0.585 μg and 0.040 8 - 0.204μg, respectively. The average recoveries of these three compounds were 99.96% ,99.24% and 100.46%, respectively. Conclusion：The method was simple, accurate, specific and would be used for the quality control of quercetin, kaemp ferol and isorhamnetin in the Ginkgo Biloba Extract.