| 甜味蛋白thaumatin表达载体的构建 |
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| 引用本文： | 赵琦,孔建强,高音.甜味蛋白thaumatin表达载体的构建[J].首都师范大学学报(自然科学版),2003,24(2):64-67. |
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| 作者姓名： | 赵琦 孔建强 高音 |
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| 作者单位： | 首都师范大学生物系,北京,100037 |
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| 基金项目： | 国家自然科学基金资助 (A3 0 0 70 488) |
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| 摘 要： | 将克隆在不同质粒上的thaumatin基因的两个片段连接起来，连接产物克隆到质粒pUC18上．测序结果表明，连接产物是一个完整的thaumatin cDNA基因．将此基因通过基因重组技术，克隆到植物表达载体pBI121中，构建了一个新的转化植物的表达载体-pBIl21-tha.
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| 关 键 词： | 甜味蛋白 thaumatin基因 基因重组 基因克隆 植物表达载体 基因工程 |
| 修稿时间： | 2002年7月2日 |
Construction of the Expressing Vector of Thaumatin |
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| Zhao Qi\ Kong Jianqiang\ Gao Yin.Construction of the Expressing Vector of Thaumatin[J].Journal of Capital Normal University(Natural Science Edition),2003,24(2):64-67. |
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| Authors: | Zhao Qi\ Kong Jianqiang\ Gao Yin |
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| Abstract: | After cheated with T\-4 ligase, the two fragments which were cloned into two different plasmids were ligated into a complete thaumatin gene. The traget gene was confirmed by sequencing. By DNA recombinatnt methods, the cDNA gene was cloned into vector pBI121.A new expressing vector\_\_pBI121\|tha was successfully constructed. |
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| Keywords: | thaumatin clone expressing vector |
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