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| 己糖激酶-磷酸葡萄糖异构酶法测定葡萄酒中还原糖的含量 | |
| 引用本文： | 杨艳娥,高红波,钟其顶,谷学新,熊正河,陈楠楠,陈凯.己糖激酶-磷酸葡萄糖异构酶法测定葡萄酒中还原糖的含量[J].首都师范大学学报(自然科学版),2011,32(4):47-50. |
| 作者姓名： | 杨艳娥 高红波 钟其顶 谷学新 熊正河 陈楠楠 陈凯 |
| 作者单位： | 1. 首都师范大学化学系,北京100048;中国食品发酵工业研究院,北京100027 2. 中国食品发酵工业研究院,北京,100027 3. 首都师范大学化学系,北京,100048 4. 赛默飞世尔科技(中国)有限公司,北京,100007 |
| 摘 要： | 建立了快速检测葡萄酒中还原糖含量的分析方法,实验表明还原糖含量在0.015～2.000g/L浓度范围内线性关系良好,相关系数为0.999 4;加标回收率(n=3)在97.56%～107.66%之间;精密度和重复性(n=10)的RSD%分别为1.26%和2.14%.该法具有无需样品前处理、特异性好、灵敏度高、测定速度快等优点,适用于葡萄酒企业对葡萄酒酿造过程控制和葡萄酒产品质量检测. |
| 关 键 词： | 还原糖 葡萄酒 酶法试剂盒 Arena自动分立式分析技术 |
Enzymatic Determination of Reducing Sugar in Wine with Automatic Discrete Analyzer |
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| Yang Yane,Gao Hongbo,Zhong Qiding,Gu Xuexin,Xiong Zhenghe,Chen Nannan,Chen Kai.Enzymatic Determination of Reducing Sugar in Wine with Automatic Discrete Analyzer[J].Journal of Capital Normal University(Natural Science Edition),2011,32(4):47-50. | |
| Authors: | Yang Yane Gao Hongbo Zhong Qiding Gu Xuexin Xiong Zhenghe Chen Nannan Chen Kai |
| Institution: | Yang Yane1,2 Gao Hongbo2 Zhong Qiding2 Gu Xuexin1 Xiong Zhenghe2 Chen Nannan1 Chen Kai3(1.Department of Chemistry,Capital Normal University,Beijing 100048,2.China National Research Institute of Food & Fermentation Industries,Beijing 100027,3.Thermo Fisher Scientific(China) Inc.,Beijing 100007) |
| Abstract: | A method was developed for rapidly analyzing the concentration of reducing sugar in wines.A suitable linear relationship was obtained between the concentrations of Reducing sugar(0.015～2.000 g/L) and the correlation coefficient was 0.999 4;the Recovery ratio(n=3) were between 97.56%～107.66%;the RSD% of precision and repeatability(n=10) were 1.26% and 2.14% respectively.This method is simple,specific,sensitive,rapid,and good for determination of Reducing sugar in wines,suitable for monitoring samples during wine-making and brewing process and quality control for the finished wine in winery. |
| Keywords: | reducing sugar wine enzymatic kits Arena Automatic Discrete Technology |
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