| 睡菜外植体愈伤组织诱导与培养初步研究 |
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| 引用本文： | 吴昌宇,华振玲,李学东.睡菜外植体愈伤组织诱导与培养初步研究[J].首都师范大学学报(自然科学版),2012(4):27-31. |
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| 作者姓名： | 吴昌宇 华振玲 李学东 |
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| 作者单位： | 北京市第八十中学;首都师范大学生命科学学院 |
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| 基金项目： | 北京市科委重大科技计划项目(D08040600580803)资助 |
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| 摘 要： | 利用睡菜带芽的嫩茎段作为外植体,切成0.5 cm长的片段,通过MS培养基和激素配比实验,筛选出了睡菜茎段愈伤组织诱导的最佳培养基配方.实验结果表明：适宜的消毒时间75%酒精30 s,升汞9 min;适宜的初代培养基为MS＋BA 1.0 mg.L^-1＋NAA 0.10 mg.L^-1;适宜的继代培养基为MS＋BA 0.5 mg.L^-1＋NAA 0.05 mg.L^-1;在培养基中添加1.5 g/L的多菌灵粉剂可以有效抑制真菌生长,诱导出愈伤组织后应在30 d内及时进行继代培养.
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| 关 键 词： | 睡菜 组织培养 愈伤组织 |
A Preliminary Study of Tissue Cultrue and Rapid Propagation on Menyanthes trifoliata L. |
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| Wu Changyu,Hua Zhenling,Li Xuedong.A Preliminary Study of Tissue Cultrue and Rapid Propagation on Menyanthes trifoliata L.[J].Journal of Capital Normal University(Natural Science Edition),2012(4):27-31. |
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| Authors: | Wu Changyu Hua Zhenling Li Xuedong |
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| Institution: | 1.Beijing No.80 Middle School,Beijing 100102;2.College of Life Science,Capital Normal University,Beijing 100048) |
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| Abstract: | The study takes fresh stem with bud from Menyanthes trifoliata as explants,then cut it into fragments about 0.5cm long.Through a series of optimization expriment for cultural medium composition of phytohormones,the best culture medium formula for callus induction of this plant’s stem fragments was selected.The results indicated that the young stem segment with buds was the best explant;the suitable sterilization time with 75% alcohol was 30s and with 1 g·L-1 HgCl2 9 min.MS+BA 1.0 mg·L-1+NAA 0.10 mg·L-1 was the proper medium for primary culture,and MS+BA 0.5 mg·L-1+NAA 0.05mg·L-1 was the proper medium for subculture.Adding 1.5 g/L of carbendazim powder to the medium containing could inhibit the growth of fungi effectively.The callus should be timely subcultured in 30 days after callus has been inducted. |
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| Keywords: | Menyanthes trifoliata tissue culture callus |
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