| 聚丙烯酰胺凝胶电泳快速显色方法的研究 |
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| 引用本文： | 刘美艳,营孙阳,张健,楚素芳.聚丙烯酰胺凝胶电泳快速显色方法的研究[J].实验室科学,2012,15(6):71-73. |
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| 作者姓名： | 刘美艳 营孙阳 张健 楚素芳 |
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| 作者单位： | 江苏师范大学 生命科学学院,江苏 徐州,221116 |
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| 基金项目： | 转基因生物新品种重大专项，江苏高校优势学科建设工程资助项目 |
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| 摘 要： | 通过对聚丙烯酰胺凝胶电泳的固定方法、时间、染色液种类、染色方法及蛋白灵敏度的研究,确定了以10%三氯乙酸为固定液,室温固定10min的固定方法;染色液为：考马斯亮蓝G-250、95%乙醇和10%磷酸,染色时间为15min;蛋白质灵敏度与考马斯亮蓝R-250相当,达到0.1μg。
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| 关 键 词： | 蛋白质 电泳 染色 考马斯亮蓝G-250 |
Study on rapid staining protocols of protein in polyacrylamide gel electrophoresis |
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| LIU Mei-yan
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YING Sun-yang
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ZHANG Jian
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CHU Su-fang.Study on rapid staining protocols of protein in polyacrylamide gel electrophoresis[J].Laboratory Science,2012,15(6):71-73. |
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| Authors: | LIU Mei-yan YING Sun-yang ZHANG Jian CHU Su-fang |
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| Institution: | (School of Life Science,Jiangsu Normal University,xuzhou 221116,China) |
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| Abstract: | The fixing methods,fixing time,the kind of dyes,staining protocols and the sensitivity of protein in polyacrylamide gel electrophoresis were studied.The fixative solution was 10% trichloroacetic acid fixing for 10min in the normal temperature.The staining solution was CBB G-250,95% ethanol,10% phosphate and the staining time was 15min.The comparable sensitivity of protein after optimization to CBB G-250 staining,which is reaching 0.1μg. |
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| Keywords: | protein electrophoresis staining CBB G-250 |
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