| 血管生成抑制素对体外培养的血管内皮细胞增殖与凋亡的影响 |
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| 引用本文： | 迟晓艳,王艳杰,侯月利.血管生成抑制素对体外培养的血管内皮细胞增殖与凋亡的影响[J].实验室科学,2011,14(2):114-117. |
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| 作者姓名： | 迟晓艳 王艳杰 侯月利 |
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| 作者单位： | 鲁东大学生命科学学院,山东烟台,264025 |
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| 摘 要： | 为研究血管生成抑制素对体外培养的血管内皮细胞生长的影响,采用MTT法观察细胞的增殖情况,利用Hoest染色和流式细胞仪检测细胞凋亡。结果发现血管生成抑制素能够抑制血管内皮细胞的增殖,其IC50为1.19 mg/L,并干扰内皮细胞的周期,出现G0/G1期阻滞。
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| 关 键 词： | 血管生成抑制素 血管生成 细胞凋亡 流式细胞术 |
Effects of angiostatin on growth and apotosis of vascular endothelial cells in vitro |
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| CHI Xiao-yan,WANG Yan-jie,HOU Yue-li.Effects of angiostatin on growth and apotosis of vascular endothelial cells in vitro[J].Laboratory Science,2011,14(2):114-117. |
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| Authors: | CHI Xiao-yan WANG Yan-jie HOU Yue-li |
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| Institution: | CHI Xiao-yan,WANG Yan-jie,HOU Yue-li(School of Life Science,Ludong University,Yantai 264025,China) |
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| Abstract: | To study the effects of angiostatin on growth of vascular endothelial cells in vitro.Thiazoyl blue tetrazolium bromide(MTT)assay is used to observe the proliferation of endothelial cells,hoest staining and flow cytometry(FCM) are used to examine apoptosis of endothelial cells.Angiostatin evidently inhibites proliferation of vascular endothelial cells,the IC50 value by MTT assay is 1.19mg/L,and the cell cycle is restrained in G0/ G1 phase. |
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| Keywords: | angiostatin angiogenesis apoptosis flow cytometry |
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