| 银杏外种皮中活性成分的分离与鉴定 |
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| 引用本文： | 王锋,李稳宏,李多伟,毛秀光,高丽雅.银杏外种皮中活性成分的分离与鉴定[J].西北大学学报,2003,33(6):689-692. |
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| 作者姓名： | 王锋 李稳宏 李多伟 毛秀光 高丽雅 |
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| 作者单位： | [1]西北大学化工学院 [2]西北大学生命科学学院，陕西西安7l0069 |
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| 基金项目： | 陕西省教育厅重大产业培育项目(EH02337)，江苏泰德银杏制品有限公司委托开发课题(2001) |
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| 摘 要： | 报道了一种通过溶剂提取，乙醚和碱液萃取，以及酸还原，由银杏外种皮为原料得到氢化白果酸、白果酚、银杏黄酮类物质的新工艺方法，利用薄层层析、高效液相色谱、红外光谱对产品进行的定性定量测量和结构分析。初提物生物测定结果表明：对几种常见菌的抑制率为60％～80％，对几种常见害虫的杀虫率70％～80%。纯化物活性测定结果表明：黄酮类物质对几种菌的抑制率均超过70％，氢化白果酸对小麦白粉茵治疗和保护作用可分别大于80%。
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| 关 键 词： | 银杏外种皮 活性成分 分离纯化 生物测定 |
| 文章编号： | 1000-274X(2003)06-689-04 |
| 修稿时间： | 2002年4月28日 |
A study on the technical process for extracting and identifying active compounds from the testa of Ginkgo biloba L |
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| WANG Feng,LI Wen-hong,LI Duo-wei,MAO Xiu-guang,GAO Li-ya.A study on the technical process for extracting and identifying active compounds from the testa of Ginkgo biloba L[J].Journal of Northwest University(Natural Science Edition),2003,33(6):689-692. |
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| Authors: | WANG Feng LI Wen-hong LI Duo-wei MAO Xiu-guang GAO Li-ya |
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| Abstract: | A new technical process for extracting active compounds of Ginkgolic phenols,acids and flavone from the testa of Ginkgo biloba L through solvent distilling aether and lye extraction as well as acid reductive was introduced. Thin-layer chromatography (TLC),High Pressure Liquid Chromatography (HPLC) and Infra-red (IR) for qualitative and quantitative determination has been adopted to test the products. The application results on biologic activity of initial extractions showed that the ratio of sterilizations is 60-80 percent and of killing insects is 70-80 percent. The application results on the activity of pure outcomes showed that flavones have the ratio of sterilizations over 70 percent and the treatment and protection of ginkgolic acid to several bacteriums are over 80 percent respectively. |
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| Keywords: | testa of Ginkgo biloba L active compounds extraction biologic activity mensuration |
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