| 鹰嘴紫云英抗甲硫氨酸变异系离体筛选及鉴定 |
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| 引用本文： | 王瑛华,侯岁稳,贾敬芬.鹰嘴紫云英抗甲硫氨酸变异系离体筛选及鉴定[J].西北大学学报,2006,36(5):773-776. |
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| 作者姓名： | 王瑛华 侯岁稳 贾敬芬 |
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| 作者单位： | 西北大学生命科学学院，兰州大学生命科学学院，西北大学生命科学学院 陕西西安710069，肇庆学院生物学系，广东肇庆526061，甘肃兰州730000，陕西西安710069 |
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| 基金项目： | 国家自然科学基金资助项目(30370697) |
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| 摘 要： | 目的离体筛选鹰嘴紫云英(Astragalus cicerL.)抗甲硫氨酸变异系。方法通过NaN3诱变处理鹰嘴紫云英(Astragalus cicerL.)的愈伤组织来筛选获得抗甲硫氨酸变异细胞系。结果获得了抗100 mmol/L甲硫氨酸变异细胞系,并分化成再生植株。结论该变异系对甲硫氨酸具有一定的抗性,并对乙硫氨酸具有交叉抗性。
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| 关 键 词： | 鹰嘴紫云英 甲硫氨酸 变异系 |
| 文章编号： | 1000-274X(2006)05-0773-04 |
| 收稿时间： | 01 10 2004 12:00AM |
| 修稿时间： | 2004年1月10日 |
In vitro selection and physiological and biochemical analyses of methionine-resistant variant in Astragalus cicer L. |
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| WANG Ying-hua,HOU Sui-wen,JIA Jing-fen.In vitro selection and physiological and biochemical analyses of methionine-resistant variant in Astragalus cicer L.[J].Journal of Northwest University(Natural Science Edition),2006,36(5):773-776. |
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| Authors: | WANG Ying-hua HOU Sui-wen JIA Jing-fen |
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| Abstract: | Aim In vitro selecting methionine variant in Astragalus cicer L.,To improve the content of methionine in Astragalus cicer L.Methods Methionine as selecture agent,using one-step positive selection isolated the variant cell line from the calluses of Astragalus cicer L.treated by NaN_3.Results The variant cell line resistant to 100 mmol/L methionine was selected.Plants were regenerated from it.Conclusion The variant cell line was resistant to methionine and ethionie. |
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| Keywords: | Astragalus cicer L methionine resistant variant |
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