沙棘幼苗雌雄植株的酶学鉴别

Studies on Seedling Early Knzymatic Diagnosis of Morphologically Unknown Sex in Hippophae Rhamnoides

Using both poiyacrylamide gel electrophoresis and disc electrophoresis methods, the peroxidase isozyme patterns of the leaves of plants of different sexes in Hippophae rhamnoides are compared. The obtained results indicate that the isozyme patterns between the male and female plant always had one more characteristic band than the male one. Examining the isozymogram of the leaves of the trees of unkmown sex, only two types of peroxidase patterns were found:one agrees with the male, the other agrees with the female, and there are no intermediate types. Experiments performed for 4 years with Hippophae rhamnoides show that peroxidase activity is associated with sexuality. Its activity is highter in the leaves of female plant than those in the male. It is considered that peroxidase activity and isoperoxidase pattern can be used as an enzymatic test to distinguish the sex of an seedling in Hippophaex rhamnoides L.