家蚕抗菌肽的一些性质及抗肿瘤活性

由Ｅ．ｃｏｌｉＫ１２Ｄ３１诱导的家蚕血淋巴中提取的抗菌肽对热稳定，高温高压处理３０分钟仍保持原有活性。抗菌肽对木瓜蛋白酶不敏感，对蛋白酶Ｅ较敏感，而对蛋白酶Ｋ和胰蛋白酶则极为敏感，抗菌肽对ＰＨ的变化较为稳定，在ＰＨ２．２－８．０的范围内活性基本不变，肿瘤模型实验观察到免疫血淋巴对宫颈瘤有明显的抑瘤作用。抗菌肽与Ｋ５６２人髓样白血病细胞混合培育一定时间后，电镜观察可看到细胞膜和细胞器发生明显变化，最

Study On the Properties and Anticancer Activities of Antibacterial Peptide

The antibacterial peptide was isolated from Bombyx mori haemolymph induced by an injection of E. colt K12D31. It was found to be thermostable, and still active through high temperature and high pressure for thirty minutes. The antibacterial peptide wasn't sensible to papain, partially sensible to proteinase E, and the most sensible to proteinase K and trypsin. The antibacterial peptide had an inhibiting activity from PH 2. 2 to PH 8. 0.The tumor model experiments showed that immune haemolymph could inhibite the growth of the cervix tumor U14 Utra-thin section technique was used to study the mechanism of the bactericidal peptide, the tumor cell membrane was injured and intracellular substances were leaked out, the cell died soon.