| 花椒挥发油的提取、分离和抗菌实验 |
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| 引用本文： | 弭向辉,龚祝南,张卫明,张锡然.花椒挥发油的提取、分离和抗菌实验[J].南京师大学报,2004,27(4):63-66. |
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| 作者姓名： | 弭向辉 龚祝南 张卫明 张锡然 |
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| 作者单位： | [1]南京师范大学生命科学学院，江苏南京210097 [2]南京野生植物综合利用研究院，江苏南京210042 |
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| 基金项目： | 国家农业领域十五攻关重点项目 .“辛香料资源的高效利用及产业化技术研究”资助项目 ( 2 0 0 1BA5 0 2B0 5 ) . |
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| 摘 要： | 研究花椒(Zanthaxylum Bungearum Maxim)挥发油的抗菌作用．超临界CO2萃取法(CO2-SFE)和乙醇回流法提取花椒挥发油及硅胶柱分离乙醇回流法所得挥发油，适当处理后共得到8个样品，用滤纸片和试管法分别测定其抗菌活性和最低抑菌浓度(MIC)．结果表明，试验中8个样品分别对8种受试菌种有不同的抑制作用．说明花椒挥发油有明显的抑菌作用；挥发油中极性相对较小的成分主要对真菌起抑制作用，而极性相对较大的样品主要对细菌起抑制作用．
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| 关 键 词： | 花椒，挥发油，抗菌，最低抑菌浓度（MIC） |
| 文章编号： | 1001-4616(2004)04-0063-04 |
| 修稿时间： | 2004年5月25日 |
Study on the Extraction,Separation and Antimicrobial Effect of Volatile Oil from Zanthoxylum Bungeanum Maxim |
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| Mi Xianghui,Gong Zhunan,Zhang Weiming,Zhang Xiran.Study on the Extraction,Separation and Antimicrobial Effect of Volatile Oil from Zanthoxylum Bungeanum Maxim[J].Journal of Nanjing Normal University(Natural Science Edition),2004,27(4):63-66. |
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| Authors: | Mi Xianghui Gong Zhunan Zhang Weiming Zhang Xiran |
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| Institution: | Mi Xianghui~1,Gong Zhunan~1,Zhang Weiming~2,Zhang Xiran~1 |
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| Abstract: | The volatile oil from Zanthoxylum Bungeanum Maxim have been associate with various pharmacological activities. Here an investigation on the antimicrobial effects of the volatile oil has been tested. The volatile oil were prepared by Supercritical CO_2 fluid extraction(SFE) and alcohol reflux respectively, and then further separated to 8 sample parts by silica-gel chromatography based their chemical solubility. Bacteria Escherichia coli, Bacillus subtilis, Staphylococcus aureus and fugi Sacctaromyces cerevisiae, Aspergillus oryzae (3042), Aspergillus niger, Phyllosticata maydis, Colletotrichum musae were used to determine their antimicrobial activity and minimum inhibitory concentration(MIC) by methods of filter paper mensuration and tube culture. The results show good antimicrobial effects. But the different parts of the volatile oil have the varied activity to 8 tested germs. The components with relatively weak polarity of the volatile oil extracted by SFE mainly inhibit fungi,while the components with relatively strong polarity of the volatile oil by alcohol reflux chiefly inhibit bacteria. |
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| Keywords: | Zanthoxylum Bungeanum Maxim volatile oil antimicrobial effect minimum inhibitory concentration (MIC) |
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