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鳡鱼群体遗传多样性的AFLP分析
引用本文:韩晓磊,徐建荣,李小蕊,伊纪峰,杨家新,许璞.鳡鱼群体遗传多样性的AFLP分析[J].南京师大学报,2009,32(1).
作者姓名:韩晓磊  徐建荣  李小蕊  伊纪峰  杨家新  许璞
作者单位:韩晓磊,许璞,Han Xiaolei,Xu Pu(南京师范大学生命科学学院,江苏,南京,210097;常熟理工学院生物与食品工程系,江苏,常熟,215500);徐建荣,Xu Jianrong(常熟理工学院生物与食品工程系,江苏,常熟,21s500);李小蕊,伊纪峰,杨家新,Li Xiaorui,Yi Jifeng,Yang Jiaxin(南京师范大学生命科学学院,江苏,南京,210097)  
基金项目:江苏省科技厅资助项目,苏州市科技局资助项目 
摘    要:利用AFLP分子标记技术对长江下游苏州段野生和野生F1代人工养殖的2个鳡鱼(Elopichthys bambusa)群体遗传多样性进行分析研究.6对选择性引物在2个群体47个个体中,共扩增出313个位点,多态位点47个.鳡鱼野生和野生F1代人工养殖2个群体的多态位点比率和Shannon's指数分别为13.42%、8.31%,0.0544、0.032 O;前者遗传多样性较后者略高.基因分化系数Gst 和Shannon's指数分析均显示2个鳡鱼群体之间出现一定遗传分化.鳡鱼UPGMA系统树有分支出现且依据群体分别聚类,表现出一定的遗传趋异.结果分析表明,鲢鱼群体的遗传多样性相对贫乏;野生F1代人工养殖群体尚未形成自己独立的遗传结构,但2个群体间已经产生了一定的遗传分化,经过较多世代的人工繁育有可能形成自己独立而稳定的遗传结构.

关 键 词:鳡鱼  遗传多样性

Analysis of Genetic Diversity of Elopichthys bambusa by AFLP Markers
Han Xiaolei,Xu Jianrong,Li Xiaorui,Yi Jifeng,Yang Jiaxin,Xu Pu.Analysis of Genetic Diversity of Elopichthys bambusa by AFLP Markers[J].Journal of Nanjing Normal University(Natural Science Edition),2009,32(1).
Authors:Han Xiaolei    Xu Jianrong  Li Xiaorui  Yi Jifeng  Yang Jiaxin  Xu Pu
Institution:1.School of Life Science;Nanjing Normal University;Nanjing 210097;China;2.Department of Biology and Food Engineering;Changshu Institute of Technology;Changshu 215500;China
Abstract:Genetic diversity of a wild populations and a cultured population of Elopichthys bambusa was investigated using amplified fragment length polymorphism(AFLP).The wild population was collected from Suzhou area of Yangtze River and the cultured population was hatchery-reared offspring of wild caught individuals from the same area.Six EcoRI and MseI primer combinations produced 47 polymorphic markers out of the total of 313 bands amplified from 47 total individuals.The proportions of polymorphic loci of the wil...
Keywords:AFLP
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