| 条斑紫菜ACEI肽结构鉴定与性能试验 |
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| 引用本文： | 何荣海,马海乐.条斑紫菜ACEI肽结构鉴定与性能试验[J].江苏大学学报(自然科学版),2012,33(4):430-434. |
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| 作者姓名： | 何荣海 马海乐 |
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| 作者单位： | 江苏大学 食品与生物工程学院,江苏 镇江 212013;江苏省农产品物理加工重点实验室,江苏 镇江 212013;江苏省农产品生物加工与分离工程技术研究中心,江苏 镇江 212013 |
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| 基金项目： | 国家自然科学基金资助项目(31071502);中国博士后基金资助项目(20100471386);江苏高校优势学科建设工程项目;南通市产学研联合创新科技计划项目(B2010008) |
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| 摘 要： | 对条斑紫菜蛋白酶解获得的血管紧张素转换酶抑制肽(ACEI)进行纯化和结构鉴定,并研究了条斑紫菜ACEI肽体外贮存稳定性和对原发性高血压大鼠的降血压作用.结果表明:通过2步反相高效液相制备色谱纯化得到2种单一组分ACEI肽,经液质联用质谱分析肽序列分别为Cys-Ser-Asn-Arg和Pro-Cys-His-Trp,二者对血管紧张素转换酶半数抑制浓度分别为24.71和5.38 μmol·L-1;条斑紫菜ACEI肽不耐高温,在中性或酸性条件下较稳定;用分子质量小于3 ku条斑紫菜ACEI肽组分对原发性高血压大鼠灌胃后有显著降血压效果,且降血压幅度随灌胃剂量增大而提高.
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| 关 键 词： | 条斑紫菜 血管紧张素转换酶抑制肽 结构鉴定 性能 蛋白质 |
Structure characterization and performance determination of Porphyra yezoensis ACEI peptides |
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| He Ronghai
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Ma Haile.Structure characterization and performance determination of Porphyra yezoensis ACEI peptides[J].Journal of Jiangsu University:Natural Science Edition,2012,33(4):430-434. |
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| Authors: | He Ronghai Ma Haile |
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| Institution: | 1,2,3(1.School of Food and Biological Engineering,Jiangsu University,Zhenjiang,Jiangsu 212013,China;2.Jiangsu Key Laboratory for Physical Processing on Agricultural Products,Zhenjiang,Jiangsu 212013,China;3.Jiangsu Province Research Center of Bio-process and Separation of Agri-products,Zhenjiang,Jiangsu 212013,China) |
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| Abstract: | Angiotensin I-converting enzyme inhibitory(ACEI) peptides extracted from Porphyra yezoensis protein were purified and identified.The stability and anti-hypertensive effect of peptides on spontaneously hypertensive rats were also investigated.After two-step reverse-phase high performance liquid chromatography purification,two ACEI peptides were achieved and identified by liquid chromatography mass spectrometry analysis as Cys-Ser-Asn-Arg and Pro-Cys-His-Trpw with 50% inhibitory concentration on Angiotensin I-converting enzyme of 24.71 μmol·L-1 and 5.38 μmol·L-1,respectively.The results indicate that Porphyra yezoensis ACEI peptides are stable under neutral or acidic condition,but with poor resistance to high temperature.The significant anti-hypertensive effect of Porphyra yezoensis ACEI peptides(MW<3 ku fraction) on spontaneously hypertensive rats was observed in oral administration test,and the blood pressure of rats decreases obviouly with the increasing of dose. |
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| Keywords: | Porphyra yezoensis ACEI peptides structure characterization capability protein |
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