| 桑黄菌诱变菌株液体发酵试验 |
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| 引用本文： | 马海乐,闫景坤,祝子坪,郑惠华,杨小明.桑黄菌诱变菌株液体发酵试验[J].江苏大学学报(自然科学版),2012,33(1):6-10. |
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| 作者姓名： | 马海乐 闫景坤 祝子坪 郑惠华 杨小明 |
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| 作者单位： | 1. 江苏大学食品与生物工程学院,江苏镇江,212013
2. 江苏安惠生物科技有限公司,江苏南通,226009 |
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| 基金项目： | 江苏高校优势学科建设工程资助项目 |
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| 摘 要： | 以激光-紫外线复合诱变筛选的桑黄菌菌株S1为研究对象,通过单因素试验初步研究了液体发酵条件,根据二次回归旋转正交组合设计确定的最佳发酵条件为:装瓶量120 mL、接种量17 mL、发酵温度26℃、摇床转速135 r·min-1,并通过验证试验得到实际菌丝产量为(24.47 ±0.80)g·L-1.在此基础上进行了小型发酵罐试验,得到的最佳发酵条件为:搅拌速度180 r·min-1、温度29℃、空气流量6 L·min-1,得到的菌丝和胞外多糖产量分别为(68.23±2.51)g和(10.23 ±0.35)g.
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| 关 键 词： | 桑黄菌 诱变菌株 液体发酵 胞外多糖 二次回归旋转正交组合设计 |
Liquid fermentation experiment of mutagenic strain from Phellinus igniarius |
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| Ma Haile
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Yan Jingkun
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Zhu Ziping
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Zheng Huihua
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Yang Xiaoming.Liquid fermentation experiment of mutagenic strain from Phellinus igniarius[J].Journal of Jiangsu University:Natural Science Edition,2012,33(1):6-10. |
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| Authors: | Ma Haile Yan Jingkun Zhu Ziping Zheng Huihua Yang Xiaoming |
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| Institution: | 1(1.College of Food Science and Biological Technology,Jiangsu University,Zhenjiang,Jiangsu 212013,China;2.Jiangsu Alphay Biological Technology Company Limited,Nantong,Jiangsu 226009,China) |
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| Abstract: | Phellinus igniarius strain S1 mutagenized with laser and UV was chosen to study the conditions of liquid fermentation by single-factor experiment.Based on quadratic orthogonal rotation combination design,the optimum conditions were determined as media amount of 120 mL with inoculum size of 17 mL,and fermentation temperature of 26 ℃ at rotate speed of 135 r·min-1.According to verification experiment,the actual mycelial yield was at the range of(24.47±0.80) g·L-1.The compact fermentor experiment was conducted based on the designed optimum conditions.The results show that the optimum fermentation conditions were with stirring speed of 180 r·min-1 at temperature of 29 ℃ with air flow of 6 L·min-1.The mycelia and expolysaccharide yields can reach the range of(68.23±2.51)g and(10.23±0.35)g,respectively. |
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| Keywords: | Phellinus igniarius mutational strain liquid fermentation expolysaccharide quadratic orthogonal rotation combination design |
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