| 血管内皮抑素的基因克隆及其在大肠杆菌中的表达 |
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| 引用本文： | 林丽珠,赵强,周炜,王光清,赵寿元,李昌本.血管内皮抑素的基因克隆及其在大肠杆菌中的表达[J].复旦学报(自然科学版),2002,41(1):106-108. |
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| 作者姓名： | 林丽珠 赵强 周炜 王光清 赵寿元 李昌本 |
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| 作者单位： | 复旦大学生命科学学院遗传工程国家重点实验室,上海,200433 |
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| 基金项目： | 国家“攀登”计划资助项目 |
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| 摘 要： | 血管内皮抑素不仅能特异性地抑制血管内皮生长，而且对血管形成和肿瘤生长也具有强烈的抑制作用。利用PCR法从人胎肝cDNA文库中克隆了人血管内皮抑素的编码序列，构建了重组质粒pRSendo，并在E.coli中表达，SDS－PAGE表明其表达产物为包涵体，此外，还对血管内皮抑素做了初步的纯化。
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| 关 键 词： | 血管内皮抑素 基因克隆 表达 |
| 文章编号： | 0427-7104(2002)01-0106-03 |
Cloning of the Endostatin Gene and Its Expression in Escherichia coli |
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| LIN Li zhu,ZHAO Qiang,ZHOU Wei,WANG Guang qing,ZHAO Shou yuan,LI Chang ben.Cloning of the Endostatin Gene and Its Expression in Escherichia coli[J].Journal of Fudan University(Natural Science),2002,41(1):106-108. |
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| Authors: | LIN Li zhu ZHAO Qiang ZHOU Wei WANG Guang qing ZHAO Shou yuan LI Chang ben |
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| Abstract: | Endostatin specifically inhibits endothelial proliferation and potently inhibits angiogenesis and tumor growth. By using PCR method, the coding sequence of endostatin was amplified from the fetal liver cDNA library. The recombinant plasmid was named as PRSendo and expressed in E. coli . SDS PAGE showed that the expression products of PRSendo existed as a form of inclusion body. In addition, this protein was preliminary purified. |
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| Keywords: | endostatin gene cloning expression |
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