| 肝癌差异表达基因HCUTAL2在酵母Pichia pastoris中的表达 |
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| 引用本文： | 黄燕,李瑶,刘建平,连福治,薛波,陈华,谢毅,毛裕民.肝癌差异表达基因HCUTAL2在酵母Pichia pastoris中的表达[J].复旦学报(自然科学版),2000,39(6):660-662. |
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| 作者姓名： | 黄燕 李瑶 刘建平 连福治 薛波 陈华 谢毅 毛裕民 |
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| 作者单位： | 复旦大学,生命科学学院,遗传学研究所,上海,200433 |
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| 基金项目： | 上海现代生物与新药产业发展基金资助项目!(98431912 1) |
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| 摘 要： | 利用正常肝组织和肝癌组织的mRNA，通过逆转录方法，将Cy3和Cy5二种荧光分别标记到2种组织的cDNA上，制备成cDNA探针，并与包含4096条各种人类基因的DNA表达谱芯片进行杂交及扫描，重复11次实验，通过计算机数据处理判定基因是否在上述2种组织中存在表达差异，从而鉴定参与肿瘤发生的基因，其中1类差异表达基因为CUTA的高度同源基因，该基因可能参与重金属离子在体的代谢，对该基因拟编码的蛋白质进行酵母表达。
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| 关 键 词： | CUTA基因 基因芯片 基因表达 肝癌差异表达基因 酵母系统 |
Exprssion of a Human CUTA Gene in Yeast Pichia pastoris |
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| HUANG Yan,LI Yao,LIU Jian-ping,LIAN Fu-zhi,XUE Bo,CHEN Hua,XIE Yi,MAO Yu-min.Exprssion of a Human CUTA Gene in Yeast Pichia pastoris[J].Journal of Fudan University(Natural Science),2000,39(6):660-662. |
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| Authors: | HUANG Yan LI Yao LIU Jian-ping LIAN Fu-zhi XUE Bo CHEN Hua XIE Yi MAO Yu-min |
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| Abstract: | The chip which contains 4 096 human genes was hybridized to Cy5 marked probes made by RT PCR with the template of the mRNA from human liver cancer while Cy3 labelled probe made of human liver mRNA was used as a control. The experiments were repeated 11 times and the results of revealed computer analysis the differentially expressed genes. One of them was a CUTA homologous gene which may be involved in heavy metal metabolism. The gene expression was performed in yeast Pichia pastoris. It is helpful to downstream functional study. |
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| Keywords: | CUTA microarray gene expression |
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