| 农杆菌介导遗传转化中辅助处理方法的改良 |
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| 引用本文： | 刘志学,马向前.农杆菌介导遗传转化中辅助处理方法的改良[J].复旦学报(自然科学版),1999,38(5):601-604. |
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| 作者姓名： | 刘志学 马向前 |
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| 作者单位： | 复旦大学遗传学研究所 |
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| 基金项目： | 国家科委八六三高科技项目，洛氏基金 |
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| 摘 要： | 在农杆菌菌液与植物材料混合培养时辅以Ｔｗｅｅｎ２０和负压处理。用ｇｕｓ报告基因的瞬间表达作为指标，与仅用Ｔｗｅｅｎ２０或仅用负压处理的样本进行了比较研究。实验结果表明：适当的负压处理可提高转化频率，而过度的负压处理会对材料造成较大的伤害，从而降低其成活率，如同时使用Ｔｗｅｅｎ２０可以减轻这种伤害。
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| 关 键 词： | 农杆菌 转化 水稻 Tween20 |
An Improved Assisting Method for Genetic Trans formation via Agrobacterium tumefaciens |
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| LIU Zhi xue,MA Xiang qian,HE Yi yuan,Xu Ya nan,YE Ming ming,TANG Ke xuan.An Improved Assisting Method for Genetic Trans formation via Agrobacterium tumefaciens[J].Journal of Fudan University(Natural Science),1999,38(5):601-604. |
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| Authors: | LIU Zhi xue MA Xiang qian HE Yi yuan Xu Ya nan YE Ming ming TANG Ke xuan |
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| Institution: | Institute of Genetics |
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| Abstract: | When the cell suspension of Agrobacterium tumefaciens was mixed and co cultured with plant cells, the mixture was disposed using Tween 20 and negative pressure simultanecously. According to the transient expression of gus report gene in the callus treated, the effect of the disposition was compared with that of using Tween 20 or negative pressure only. The results showed that appropriate treatment could increase the transformation frequency and survival rate while over treatment harmed plant cells and decreased the survival rate. Addition of Tween 20 while using negative pressure could decrease the harmness caused by the over treatment. |
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| Keywords: | Agrobacterium tumefaciens transformation rice negative pressure Tween 20 |
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