| 加拿大一枝黄花挥发油成分及其抗菌活性 |
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| 引用本文： | 张劲松,李博,陈家宽,周铜水.加拿大一枝黄花挥发油成分及其抗菌活性[J].复旦学报(自然科学版),2006,45(3):412-416. |
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| 作者姓名： | 张劲松 李博 陈家宽 周铜水 |
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| 作者单位： | 复旦大学,生命科学学院,天然药物研究中心,上海,200433 |
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| 摘 要： | 为研究加拿大一枝黄花挥发油的化学成分及其抗菌活性,采用水蒸气蒸馏法从上海江湾机场9月所产加拿大一枝黄花中提取挥发油,并用GC-MS技术结合计算机检索对其化学成分进行分析鉴定,用色谱峰面积归一化法计算各组分的相对质量百分数.现共鉴定了加拿大一枝黄花挥发油的28个成分,是总挥发油的85.88%,其主要成分为大香叶烯D(Germacrane D),D-柠檬烯(D-Limonene).另采用平板打孔法,测试了加拿大一枝黄花挥发油的抗菌活性.结果表明,加拿大一枝黄花挥发油对大肠杆菌和枯草芽孢杆菌具有显著的抑制作用,对金黄色葡萄球菌作用不明显.大香叶烯D(Germacrene D)是其主要的抗菌活性成分.
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| 关 键 词： | 加拿大一枝黄花 菊科 挥发油 GC-MS 平板打孔法 抗菌活性 |
| 文章编号： | 0427-7104（2006）03-0412-05 |
| 收稿时间： | 2005-04-25 |
| 修稿时间： | 2005-04-25 |
Chemical Constituents and Antimicrobial Activity of Volatile Oil from Solidago canadensis L. |
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| ZHANG Jin-song,LI Bo,CHEN Jia-kuan,ZHOU Tong-shui.Chemical Constituents and Antimicrobial Activity of Volatile Oil from Solidago canadensis L.[J].Journal of Fudan University(Natural Science),2006,45(3):412-416. |
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| Authors: | ZHANG Jin-song LI Bo CHEN Jia-kuan ZHOU Tong-shui |
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| Institution: | Research Center of Natural Products, College of Life Sciences, Fudan University, Shanghai 200433, China |
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| Abstract: | The chemical components of volatile oil extracted by stream distillation from Solidago canadensis L.were analyzed and identified by GC-MS.The relative content of each component was determined by area normalization.49 peaks were separated and 28 compounds were identified representing 85.88% of the total contents.The main volatile oil constituents of Solidago canadensis L.in Shanghai were Germacrane D and D-Limonene.The disk diffusion method was applied into testing the antimicrobial activity of this volatile oil from Solidago canadensis L.The volatile oil showed strong inhibition against Esherichia coli and Bacillus subtilis,but weak inhibition against Staphylococcus aureus.Germacrane D was found to be the main antimicrobial component. |
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| Keywords: | Solidago canadensis L compositae volatile oil GC-MS disk diffusion method antimicrobial activity |
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