| 浙江有尾两栖动物分类与演化的RAPD研究 |
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| 引用本文： | 王世平.浙江有尾两栖动物分类与演化的RAPD研究[J].复旦学报(自然科学版),1998,37(4):485-490. |
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| 作者姓名： | 王世平 |
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| 作者单位： | 复旦大学(王世平，陈燃，黄正一，杨树青，毛裕民)，杭州师范学院(顾辉清)，复旦大学环境和资源生物学系(董惠琴，杨琰云) |
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| 摘 要： | 选用２０个随机短引物和６个随机长引物对来自３个科５个属７个种的有尾两栖动物进行ＲＡＰＤ分析，１２个引物得到清晰的ＤＮＡ扩增图谱，共计２０７条多态性片段。
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| 关 键 词： | 有尾两栖动物 分类 演化 系统树 RAPD |
Taxonomic and Evolutionary Studies Using RAPD on Zhejiang Tailed Amphibians |
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| Wang Shiping, Chen Ran, Huang Zhengyi, Yang Shuqing, Gu Huiqing, Mao Yumin.Taxonomic and Evolutionary Studies Using RAPD on Zhejiang Tailed Amphibians[J].Journal of Fudan University(Natural Science),1998,37(4):485-490. |
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| Authors: | Wang Shiping Chen Ran Huang Zhengyi Yang Shuqing Gu Huiqing Mao Yumin |
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| Abstract: | Twenty short arbitrary primers (10 nt) and six long ones (20 nt) were used in RAPD analysis for Zhejiang tailed amphibians of seven species. Twelve primers could generate clear amplified patterns. Two hundred and seven polymorphic bands were obtained.UPGMA method was used to construct phylogenetic tree by computer software. The result showed that Hynobius chinensis and Hynobius sp. in Zhoushan had the smallest genetic distance, and Andrias davidianus was the most different from others. |
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| Keywords: | tailed amphibians taxonomy evolution phylogenetic tree RAPD |
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