| RBM13 cDNA的克隆及其表达谱分析 |
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| 引用本文： | 周宗祥,徐坚,顾少华,鲍烨敏,曾立,应康,谢毅,毛裕民.RBM13 cDNA的克隆及其表达谱分析[J].复旦学报(自然科学版),2000,39(6):583-587. |
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| 作者姓名： | 周宗祥 徐坚 顾少华 鲍烨敏 曾立 应康 谢毅 毛裕民 |
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| 作者单位： | 复旦大学,生命科学学院,遗传学研究所,上海,200433 |
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| 基金项目： | 上海市现代生物与新药产业发展基金资助项目!( 984 31 91 2 1 ) |
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| 摘 要： | 从人胎脑cDNA文库中克隆到一条长3479bp的cDNA，它含一个长903bp的开放阅读框架，拟编码一个300个氨基酸的蛋白质，其分子质量为35397u，等电点为5．35，与酵母MAK16蛋白的同源性为41％，该编码蛋白有一个双侧核定位信号motif和一个RNA结合motif，将这一新cDNA序列推导的蛋白命名为RNA结合基序蛋白13（RBM13），基因定名为RBM13，用RBM13基因的cDNA探针进行Northern杂交，检测到3．6kb和2．2kb二种长度的转录本。在心脏，骨骼肌，肾脏和肝脏中有较高表达。
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| 关 键 词： | RNA结合motif CDNA克隆 Northern杂交 表达谱 RNA结合蛋白 |
Cloning and Northern Blot Analysis of RBM13 Gene |
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| ZHOU Zong-xiang,XU Jian,GU Shao-hua,BAO Ye-min,ZENG Li,YING Kang,XIE Yi,MAO Yu-min.Cloning and Northern Blot Analysis of RBM13 Gene[J].Journal of Fudan University(Natural Science),2000,39(6):583-587. |
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| Authors: | ZHOU Zong-xiang XU Jian GU Shao-hua BAO Ye-min ZENG Li YING Kang XIE Yi MAO Yu-min |
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| Abstract: | A full length cDNA with 3 479 bp was cloned from a fetal brain cDNA library. It contains an open reading frame encoding a 300 amino acid protein with a calculated molecular mass of 35 379 u and a pI of 5.35. The deduced protein, which was named as RNA binding motif protein 13 (RBM13), shows 41 % identity to MAK16 protein of S. cerevisiae . It contained the signals of a bipartite nuclear localization and an eukaryotic putative RNA binding region RNP 1. Northern blot analysis showed that two sizes of human RBM 13 messenger RNAs (3.6 kb and 2.2 kb) were widely appeared, with highest levels in heart, skeletal muscle, kidney and liver. |
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| Keywords: | RNA binding motif cDNA clone Northern blot |
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