| 一个新的PMA诱导相关基因在Pichia pastoris酵母系统中的表达 |
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| 引用本文： | 黄燕,吴超群,和桂芬,刘建平,连福治,薛波,陈华,谢毅,毛裕民.一个新的PMA诱导相关基因在Pichia pastoris酵母系统中的表达[J].复旦学报(自然科学版),2000,39(6):656-659. |
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| 作者姓名： | 黄燕 吴超群 和桂芬 刘建平 连福治 薛波 陈华 谢毅 毛裕民 |
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| 作者单位： | 复旦大学,生命科学学院,遗传学研究所,上海,200433 |
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| 基金项目： | 上海市现代生物与新药产业发展基金资助项目!(98431912 1) |
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| 摘 要： | 利用包含 40 96条各种人类基因的DNA芯片研究了代谢增强剂PMA(phorbolmyristateacetate)激活的人单核巨噬细胞HTP 1的早期应答基因 ,根据已有的旧基因信息 ,实验结果基本反映了人单核巨噬细胞早期应答基因的表达谱 .选取其中一条候选早期应答基因为后续研究对象 ,并进行蛋白质的酵母表达 ,为下游的基因功能研究奠定一定的基础 .
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| 关 键 词： | PMA刺激 基因芯片 基因表达 |
Expression in Pichia pastoris of a New PMA-induced Associated Gene |
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| HUANG Yan,WU Chao-qun,HE Gui-fen,LIU Jian-ping,LIAN Fu-zhi,XUE Bo,CHEN Hua,XIE Yi,MAO Yu-ming.Expression in Pichia pastoris of a New PMA-induced Associated Gene[J].Journal of Fudan University(Natural Science),2000,39(6):656-659. |
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| Authors: | HUANG Yan WU Chao-qun HE Gui-fen LIU Jian-ping LIAN Fu-zhi XUE Bo CHEN Hua XIE Yi MAO Yu-ming |
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| Abstract: | The response of monocyte derived macrophage cells to PMA was explored with a complementary DNA microarray representing about 4 096 different human genes. Globally gene expression profile of THP 1 cell early responsing to PMA was confirmed by old gene expression information. A new gene which was a putative ERG was selected and expressed in yeast ( pichia pastoris ) and it is helpful to study the function of the downstream genes. |
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| Keywords: | phorbol myristate acetate microarray gene expression |
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